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Published on: September 22, 2013
Native fluorescence spectroscopy reveals spectral differences among prostate cancer cell lines with different risk
Yang Pu1, Jianpeng Xue, Wubao Wang
1City College of the City University of New York, Institute for Ultrafast Spectroscopy and Lasers, Department of Physics, New York, New York 10031, USA.
Abstract:
The spectral changes of native fluorophores among normal fibroblasts and cancer cell lines of different metastatic ability are investigated by fluorescence spectroscopy. The normal (fibroblast), moderately metastatic (DU-145), and advanced metastatic (PC-3) cell lines were each selectively excited at 300 nm, and their fluorescence emission spectra are analyzed using principal component analysis to explore the differences of the relative contents of tryptophan and reduced nicotinamide adenine dinucleotide in these cell lines. The results show that the tryptophan emission featured predominantly in the fluorescence spectra of the advanced metastatic cancer cells in comparison with the moderately metastatic cancer and normal cells.
