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Updated: May 9, 2026

Analysis of Protein Import into Chloroplasts Isolated from Stressed Plants
Published on: November 1, 2016
Functional analysis of heterotrimeric G proteins in chloroplast development in Arabidopsis
1National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
Abstract:
Functional analysis of G-proteins has been extensively carried out using their over-expressing lines and knockout mutants in plants. Since α subunit exists in an active or inactive form, overexpressing α subunit does not mean that G-protein signaling pathways are activated in the transgenic lines. Ectopic expression of the constitutively active form of the α subunit will magnify a role of G-protein signaling pathways in plant growth and development, and ultimately yield phenotypes. Here, we describe the method to study function of G-proteins in chloroplast development using the constitutively active form of the α subunit in Arabidopsis.
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