Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The Effects of Population Density on the Incidence of Developmental Deformities in Chemosensory Organs of Tobacco Hornworm Larvae (Lepidoptera: Sphingidae).

Journal of insect science (Online)·2020

Same author

Reflections from a Patient and Carer on Involvement in Research and Integrating Care in the Health System.

International journal of integrated care·2017

Same author

Off-axis detection and characterization of laser beams in the maritime atmosphere.

Applied optics·2011

Same author

Effects of underwater turbulence on laser beam propagation and coupling into single-mode optical fiber.

Applied optics·2010

Same author

Humidity detection and hygropreference behavior in larvae of the tobacco hornworm, Manduca sexta.

Journal of insect science (Online)·2010

Same author

Laser propagation at 1.56 microm and 3.60 microm in maritime environments.

Applied optics·2009

Search research articles

Related Experiment Video

Updated: May 9, 2026

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

Published on: October 31, 2015

Off-axis laser beam imaging and characterization with two cameras.

Frank Hanson¹, Ike Bendall

¹AUSGAR Technologies, Inc., San Diego, California 92131, USA. hansonfe@att.net

|August 6, 2013

Summary

Researchers used two cameras to image laser beam scattering in the atmosphere. This method precisely reconstructs laser beam position and orientation, overcoming single-camera ambiguity for atmospheric optics applications.

More Related Videos

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

Published on: August 4, 2018

Characterization of SiN Integrated Optical Phased Arrays on a Wafer-Scale Test Station

Characterization of SiN Integrated Optical Phased Arrays on a Wafer-Scale Test Station

Published on: April 1, 2020

Related Experiment Videos

Last Updated: May 9, 2026

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

Published on: October 31, 2015

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

Published on: August 4, 2018

Characterization of SiN Integrated Optical Phased Arrays on a Wafer-Scale Test Station

Characterization of SiN Integrated Optical Phased Arrays on a Wafer-Scale Test Station

Published on: April 1, 2020

Area of Science:

Optics
Atmospheric Physics
Laser Technology

Background:

Off-axis scattering of laser beams is a phenomenon in atmospheric optics.
Understanding laser beam propagation is crucial for various applications, including remote sensing and optical communication.
Previous methods for determining beam position and orientation from scattered light were limited.

Purpose of the Study:

To develop a method for reconstructing the position and orientation of laser beams in the atmosphere using off-axis scattering.
To analyze the theoretical basis and experimental validation of this imaging technique.
To address the ambiguity inherent in single-camera measurements.

Main Methods:

Imaging off-axis scattered laser light using two separated cameras.
Developing a theoretical framework for image analysis.
Utilizing the intersection of ambiguity planes from two camera views to uniquely determine beam parameters.
Employing relative radiance measurements for beam direction determination when ambiguity planes are nearly parallel.

Main Results:

Demonstrated that images of scattered laser light can be used to reconstruct beam position and orientation.
Showed that a single camera image provides information only within an ambiguity plane.
Confirmed that the intersection of ambiguity planes from two cameras uniquely determines the beam's spatial characteristics.
Validated an independent method using relative radiance for beam direction when planes are nearly parallel.

Conclusions:

The proposed two-camera imaging method accurately reconstructs laser beam position and orientation in atmospheric conditions.
This technique effectively resolves the inherent ambiguity of single-camera measurements.
The relative radiance method offers a complementary approach for determining beam direction in specific geometric configurations.