Quorus bioreactor: a new perfusion-based technology for microbial cultivation

Area of Science:

• Biotechnology
• Bioprocess Engineering
• Cell Culture Technology

Background:

• Perfusion-based cultivation is crucial for biomanufacturing, enabling continuous cell growth and product formation.
• Traditional bioreactors face limitations with certain non-traditional production cell types.
• Advancements in single-use systems offer potential for improved bioprocess flexibility and reduced contamination risk.

Purpose of the Study:

• To review existing perfusion cultivation solutions in biomanufacturing.
• To introduce the novel single-use Quorus Bioreactor system.
• To evaluate the design, process control, and productivity of the Quorus Bioreactor for non-traditional cell cultivation.

Main Methods:

• Review of literature on perfusion bioreactor systems.
• Description of the Quorus Bioreactor's design features and single-use components.
• Implementation of process control strategies for cell cultivation.
• Experimental studies using Aspergillus niger and Lactococcus lactis as model organisms.

Main Results:

• The Quorus Bioreactor facilitates efficient cultivation of non-traditional cell types.
• Demonstrated process flexibility across different microbial species.
• Achieved high efficiency and scalability in biomanufacturing applications.
• Case studies confirmed the bioreactor's performance with Aspergillus niger and Lactococcus lactis.

Conclusions:

• The Quorus Bioreactor represents an innovative solution for perfusion-based biomanufacturing.
• Its design enables efficient and scalable cultivation of diverse cell types.
• The system offers significant advantages in process flexibility and productivity for bioprocess development.