Choline metabolic profiling by magnetic resonance spectroscopy
Egidio Iorio1, Alessandro Ricci, Maria Elena Pisanu
1Department of Cell Biology and Neurosciences, Istituto Superiore di Sanità, Rome, Italy.
Methods in Molecular Biology (Clifton, N.J.)
|August 6, 2013
Summary
Cancer cells reprogram metabolism, including phosphatidylcholine pathways. This study uses magnetic resonance spectroscopy (MRS) to measure enzyme activity linked to phosphocholine accumulation in ovarian cancer.
Area of Science:
- Biochemistry
- Oncology
- Metabolomics
Background:
- Cancer progression involves metabolic reprogramming.
- Phosphatidylcholine metabolism is crucial in ovarian cancer.
- Magnetic resonance spectroscopy (MRS) offers noninvasive metabolic monitoring.
Purpose of the Study:
- To develop methods for assaying key enzyme activity in phosphatidylcholine metabolism.
- To investigate phosphocholine accumulation in ovarian cancer cells.
- To utilize high-resolution (1)H and (31)P MRS for these analyses.
Main Methods:
- High-resolution (1)H and (31)P magnetic resonance spectroscopy (MRS).
- Analysis of cell lysates and cytosolic preparations.
- Assaying enzyme activities within the phosphatidylcholine metabolic pathway.
Main Results:
- Established comprehensive methods for enzyme activity assays.
- Quantified enzyme activities related to phosphocholine accumulation.
- Demonstrated the utility of MRS in studying cancer metabolism.
Conclusions:
- The developed MRS methods enable detailed investigation of cancer-specific metabolic pathways.
- This approach aids in understanding the role of phosphatidylcholine metabolism in ovarian cancer progression.
- Provides a foundation for further research into metabolic targets for cancer therapy.
Related Concept Videos
01:25Applications Of NMR In Biology
Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
The...
The...
01:33Proteomics
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...