Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
Small interfering RNAs (siRNA)02:30

Small interfering RNAs (siRNA)

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Physicochemical design of nanobiomaterials in oncology: structure-to-function principles for sequential transport and delivery.

Frontiers in oncology·2026
Same author

Exosome-Associated MTA1 in Circulation Is Elevated During Breast Cancer Progression.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2025
Same author

Advancing Medical Applications of Cancer Nanotechnology: Highlighting Two Decades of the NCI'S Nanotechnology Characterization Laboratory Service to the Research Community.

Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology·2025
Same author

Surgical delay-associated mortality risk varies by subtype in loco-regional breast cancer patients in SEER-Medicare.

Breast cancer research : BCR·2024
Same author

A Cyclic Peptide Targets Glioblastoma by Binding to Aberrantly Exposed SNAP25.

Molecular pharmaceutics·2024
Same author

A CYCLIC PEPTIDE TARGETS GLIOBLASTOMA BY BINDING TO ABERRANTLY EXPOSED SNAP25.

bioRxiv : the preprint server for biology·2024

Related Experiment Video

Updated: May 9, 2026

Porous Silicon Microparticles for Delivery of siRNA Therapeutics
08:31

Porous Silicon Microparticles for Delivery of siRNA Therapeutics

Published on: January 15, 2015

Mesoporous silicon particles for sustained gene silencing.

Nafis Hasan1, Aman Mann, Mauro Ferrari

  • 1Thomas Jefferson University, Philadelphia, PA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|August 6, 2013
PubMed
Summary
This summary is machine-generated.

This study presents a novel RNA interference (RNAi) delivery system using mesoporous silicon particles to load liposomes with small interfering RNA (siRNA). This approach achieved sustained gene silencing and reduced ovarian cancer tumor growth in mice without toxicity.

More Related Videos

Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery
08:53

Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery

Published on: April 16, 2019

Predicting Gene Silencing Through the Spatiotemporal Control of siRNA Release from Photo-responsive Polymeric Nanocarriers
11:53

Predicting Gene Silencing Through the Spatiotemporal Control of siRNA Release from Photo-responsive Polymeric Nanocarriers

Published on: July 21, 2017

Related Experiment Videos

Last Updated: May 9, 2026

Porous Silicon Microparticles for Delivery of siRNA Therapeutics
08:31

Porous Silicon Microparticles for Delivery of siRNA Therapeutics

Published on: January 15, 2015

Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery
08:53

Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery

Published on: April 16, 2019

Predicting Gene Silencing Through the Spatiotemporal Control of siRNA Release from Photo-responsive Polymeric Nanocarriers
11:53

Predicting Gene Silencing Through the Spatiotemporal Control of siRNA Release from Photo-responsive Polymeric Nanocarriers

Published on: July 21, 2017

Area of Science:

  • Biotechnology
  • Nanotechnology
  • Oncology

Background:

  • RNA interference (RNAi) offers a promising strategy for silencing oncogenes.
  • In vivo RNAi delivery faces challenges in safety, efficiency, and duration.
  • Targeting oncoproteins is crucial for cancer cell survival and therapeutic intervention.

Purpose of the Study:

  • To develop a novel, safe, and efficient in vivo RNAi delivery system.
  • To achieve sustained gene silencing for ovarian cancer treatment.
  • To evaluate the efficacy of mesoporous silicon particles loaded with liposomal siRNA against ovarian cancer.

Main Methods:

  • Utilized mesoporous silicon particles loaded with liposomes containing small interfering RNA (siRNA).
  • Targeted siRNA against an oncoprotein critical for cancer cell survival.
  • Administered the delivery system intravenously in orthotopic mouse models of ovarian cancer.

Main Results:

  • Achieved sustained gene silencing for at least 3 weeks.
  • Demonstrated a substantial reduction in tumor growth.
  • Observed no overt toxicities in the treated mouse models.

Conclusions:

  • Mesoporous silicon particles loaded with liposomal siRNA provide a safe and effective platform for in vivo RNAi delivery.
  • This novel approach holds potential for sustained gene silencing and ovarian cancer therapy.
  • A single intravenous administration showed significant therapeutic effects in preclinical models.