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Related Concept Videos

Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as  cells...
DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
SDS-PAGE01:27

SDS-PAGE

Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...
Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

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Related Experiment Video

Updated: May 9, 2026

Denaturing Gradient Gel Electrophoresis (DGGE)
10:52

Denaturing Gradient Gel Electrophoresis (DGGE)

Published on: February 25, 2007

Denaturing gradient gel electrophoresis (DGGE).

Fiona Strathdee1, Andrew Free

  • 1Institute of Cell Biology, University of Edinburgh, Edinburgh, UK.

Methods in Molecular Biology (Clifton, N.J.)
|August 6, 2013
PubMed
Summary

Denaturing Gradient Gel Electrophoresis (DGGE) separates DNA fragments by melting point. This method identifies genetic variations and analyzes microbial communities using 16S rRNA genes.

Area of Science:

  • Molecular Biology
  • Genetics
  • Microbiology

Background:

  • Denaturing Gradient Gel Electrophoresis (DGGE) is a molecular technique for DNA fragment separation.
  • DGGE separates DNA based on melting properties, useful for identifying single-nucleotide polymorphisms (SNPs).
  • It serves as a molecular fingerprinting method for complex microbial ecosystems, often coupled with 16S rRNA gene amplification.

Purpose of the Study:

  • To describe the principles of Denaturing Gradient Gel Electrophoresis (DGGE).
  • To provide an optimized protocol for DGGE application.
  • To demonstrate DGGE's utility in fingerprinting bacterial 16S rRNA gene fragments.

Main Methods:

  • Utilizing a chemical denaturant gradient to induce partial DNA strand separation.

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A Nonsequencing Approach for the Rapid Detection of RNA Editing
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A Nonsequencing Approach for the Rapid Detection of RNA Editing

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Agarose Gel Electrophoresis for the Separation of DNA Fragments
07:10

Agarose Gel Electrophoresis for the Separation of DNA Fragments

Published on: April 20, 2012

Related Experiment Videos

Last Updated: May 9, 2026

Denaturing Gradient Gel Electrophoresis (DGGE)
10:52

Denaturing Gradient Gel Electrophoresis (DGGE)

Published on: February 25, 2007

A Nonsequencing Approach for the Rapid Detection of RNA Editing
08:50

A Nonsequencing Approach for the Rapid Detection of RNA Editing

Published on: April 21, 2022

Agarose Gel Electrophoresis for the Separation of DNA Fragments
07:10

Agarose Gel Electrophoresis for the Separation of DNA Fragments

Published on: April 20, 2012

  • Employing gel electrophoresis to separate DNA fragments based on their unique melting points.
  • Optimizing protocols for analyzing 200-300 bp fragments of bacterial 16S rRNA genes.

    • Main Results:

    • Detailed explanation of DGGE principles and DNA melting behavior.
    • Presentation of an example protocol for DGGE analysis.
    • Demonstration of DGGE's effectiveness for fingerprinting specific DNA fragments.

    Conclusions:

    • DGGE is a valuable technique for DNA fragment analysis and genetic variation identification.
    • The described protocol facilitates the application of DGGE for microbial community fingerprinting.
    • DGGE offers an alternative to DNA sequencing for certain genetic analyses.