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Analysis of DNA-protein interactions using PAGE: band-shift assays
1Centre for Integrative Physiology, School of Biomedical Sciences, University of Edinburgh, Edinburgh, UK.
Methods in Molecular Biology (Clifton, N.J.)
|August 6, 2013
Abstract:
The band-shift assay using polyacrylamide gel electrophoresis is a powerful technique used to investigate DNA-protein interactions. The basis of the method is the separation of free DNA from DNA-protein complexes by virtue of differences in charge, size, and shape. The band-shift assay can be used to determine thermodynamic and kinetic binding constants and also to analyze the composition and stoichiometries of DNA-protein complexes.

