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Sequential Shh expression in the development of the mouse upper functional incisor.

Maria Hovorakova¹, Lucie Smrckova, Herve Lesot

¹Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic.

Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution

|August 6, 2013

Summary

Mouse incisor development relies on precise sonic hedgehog (Shh) signaling. This study details Shh expression patterns in wild-type mouse upper incisors, revealing insights into early tooth germ formation and evolutionary suppression.

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Area of Science:

Developmental biology
Craniofacial development
Mammalian organogenesis

Background:

Mouse incisors are crucial models for organ development studies.
Understanding normogenesis is key to interpreting mutant mouse data.
Detailed knowledge of wild-type incisor development is essential.

Purpose of the Study:

To investigate the early development of the upper incisor in wild-type mice.
To map the temporal-spatial expression patterns of sonic hedgehog (Shh).
To provide a comprehensive dataset for normal mouse incisor development.

Main Methods:

Histology
Whole mount in situ hybridization
Computer-aided 3D reconstructions
Fluorescent microscopy

Main Results:

Identified distinct Shh expression domains during prenatal development (ED 11.5-14.5).
Observed an anterior Shh domain related to rudimentary tooth development.
Detected a posterior Shh domain at ED 13.5, correlating with the functional incisor germ.

Conclusions:

The temporal-spatial Shh pattern reflects dual tooth generation development.
Anterior Shh expression indicates evolutionarily suppressed tooth development.
Posterior Shh expression at ED 13.5 marks the prospective functional upper incisor.