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Updated: May 9, 2026

Identification of a Murine Erythroblast Subpopulation Enriched in Enucleating Events by Multi-spectral Imaging Flow Cytometry
Published on: June 6, 2014
Analysis of primitive erythroid cell proliferation and enucleation using a cyan fluorescent reporter in transgenic
Andrei M Vacaru1, Joan Isern, Stuart T Fraser
1Department of Medicine, The Icahn School of Medicine at Mount Sinai, New York, New York; The Tisch Cancer Institute, The Icahn School of Medicine at Mount Sinai, New York, New York.
Abstract:
Primitive erythropoiesis is a vital process for mammalian embryonic development. Here we report the generation and characterization of a new transgenic mouse line that expresses a histone H2B-CFP fusion protein in the nuclei of primitive erythroid cells. We demonstrate the potential of this ε-globin-histone H2B-CFP line for multicolor imaging and flow cytometry analysis. The ε-globin-H2B-CFP line was used to analyze the cell cycle distribution and proliferation of CFP-expressing primitive erythroblasts from E8.5-E13.5. We also evaluated phagocytosis of extruded CFP-positive nuclei by macrophages in fetal liver and placenta. The ε-globin-H2B-CFP transgenic mouse line adds to the available tools for studying the development of the primitive erythroid lineage.

