Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR activation may...
T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Optineurin Shapes Basal and LPS-Induced Transcriptomes in BV2 Microglia.

International journal of molecular sciences·2025
Same author

T cell-derived IFN-γ Suppresses T Follicular Helper Cell Differentiation and Antibody Responses.

bioRxiv : the preprint server for biology·2025
Same author

Interleukin-1α alters megakaryocyte maturation, promotes emperipolesis, and induces a distinct proteomic profile.

Journal of thrombosis and haemostasis : JTH·2025
Same author

Beneficial Effect of Olive Leaf Extract as an Adjunct to Standard Antifungal Therapy in Treating <i>Candida</i>-Related Oral Diseases.

International journal of molecular sciences·2025
Same author

CD4<sup>+</sup> T cells license Kupffer cells to reverse CD8<sup>+</sup> T cell dysfunction induced by hepatocellular priming.

Nature immunology·2025
Same author

T-cell-derived IFN-γ suppresses T follicular helper cell differentiation and antibody responses.

The EMBO journal·2025
Same journal

Epigenetic control of p53 activity in regulatory T cells maintains their identity to prevent inflammation.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

Inflammatory genital strain of Chlamydia trachomatis elicits a Th17 immune response.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

The scaffolding protein AKAP79/150 shapes innate immune responses to allergen.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

Optineurin restrains IL-17-associated neuroinflammation in trigeminal ganglia to preserve sensory function after ocular HSV-1 infection.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

Crystal structure and immune single-cell atlas provide insights into the functional divergence of type I IFNs in fish.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

Complement C3 deficiency increases the effector and cytotoxic functions of NK cells and suppresses tumor growth.

Journal of immunology (Baltimore, Md. : 1950)·2026
See all related articles

Related Experiment Video

Updated: May 9, 2026

Identification of Mediators of T-cell Receptor Signaling via the Screening of Chemical Inhibitor Libraries
08:49

Identification of Mediators of T-cell Receptor Signaling via the Screening of Chemical Inhibitor Libraries

Published on: January 22, 2019

CD70 is downregulated by interaction with CD27.

Mirela Kuka1, Ivana Munitic, Maria Letizia Giardino Torchia

  • 1Laboratory of Immune Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.

Journal of Immunology (Baltimore, Md. : 1950)
|August 6, 2013
PubMed
Summary
This summary is machine-generated.

The CD70-CD27 pathway regulates T cell responses. CD70 signaling self-limits its own expression through reverse signals, preventing excessive immune activation and autoimmunity.

More Related Videos

The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity
08:26

The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity

Published on: December 14, 2016

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes
09:40

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes

Published on: September 28, 2018

Related Experiment Videos

Last Updated: May 9, 2026

Identification of Mediators of T-cell Receptor Signaling via the Screening of Chemical Inhibitor Libraries
08:49

Identification of Mediators of T-cell Receptor Signaling via the Screening of Chemical Inhibitor Libraries

Published on: January 22, 2019

The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity
08:26

The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity

Published on: December 14, 2016

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes
09:40

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes

Published on: September 28, 2018

Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Biology

Background:

  • The CD70-CD27 interaction is crucial for T cell responses, influencing immunity and autoimmunity.
  • Cell surface CD70 expression on activated immune cells like dendritic cells (DCs), T cells, and B cells is tightly regulated.

Purpose of the Study:

  • To investigate additional regulatory mechanisms of CD70 expression beyond its surface presentation on activated cells.
  • To understand how CD70-mediated signaling impacts its own levels and function.

Main Methods:

  • Analysis of CD70 recycling in immature DCs.
  • Assessment of surface CD70 levels in CD27-deficient mice and using blocking antibodies.
  • Evaluation of CD70 transcription and protein levels following CD70-CD27 engagement.

Main Results:

  • Immature DCs possess an intracellular CD70 pool that recycles to the plasma membrane.
  • Higher surface CD70 levels were observed in CD27-deficient mice and when CD70-CD27 interaction was blocked.
  • CD70 engagement by CD27 led to downregulation of CD70 transcription and protein.

Conclusions:

  • A second layer of CD70 regulation exists, involving intracellular pools and reverse signaling.
  • CD70-mediated reverse signals provide a self-regulatory mechanism, limiting CD70 expression and costimulatory potential upon receptor binding.