Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Matrix Proteoglycans and Glycoproteins01:21

Matrix Proteoglycans and Glycoproteins

Proteoglycans are extensively glycosylated proteins, commonly found in the extracellular matrix, interwoven with collagen fibers. Hyaline cartilage, the most common type of cartilage in the body, consists of short and dispersed collagen fibers associated with large amounts of proteoglycans. These proteoglycans have long negative charges that attract cations, which in turn attract water molecules. This influx of ions and water molecules swells up the proteoglycan like a water-soaked gel that can...
Role of Matrix Metalloproteases in Degradation of ECM01:23

Role of Matrix Metalloproteases in Degradation of ECM

Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult body.
A...
Essential Minerals for Bone Health01:31

Essential Minerals for Bone Health

The minerals contained in all of the food we consume are essential for our organ systems. However, certain essential minerals, such as calcium, phosphorus, magnesium, manganese, and fluoride, largely affect bone health.
Calcium and Phosphorus
Calcium is a critical component of bones, especially in the form of calcium phosphate and calcium carbonate. Since the body cannot make calcium, it must be obtained from the diet. However, calcium cannot be absorbed from the small intestine without...
Overview of Cell-Matrix Interactions01:24

Overview of Cell-Matrix Interactions

The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...
The Extracellular Matrix01:29

The Extracellular Matrix

Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
The Extracellular Matrix01:42

The Extracellular Matrix

Overview

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rapid bone formation and remodeling via vascular infiltration of a hydroxyapatite/collagen nanocomposite beneath the calvarial periosteum.

Biomaterials and biosystems·2026
Same author

Computed Tomography Analysis of Craniofacial Features in Japanese Patients With Cleidocranial Dysplasia.

Congenital anomalies·2026
Same author

Human pain transcriptomics: lessons learned so far.

Pain reports·2026
Same author

Improvement of the Respiratory Function in a Patient With Mandibular Retrognathia and Hyperdivergent Skeletal Pattern With Condylar Resorption by Le Fort I Osteotomy and Genioplasty.

Case reports in dentistry·2025
Same author

Store-operated Ca<sup>2+</sup> entry mediated by STIM1 and STIM2 regulates salivary secretion and KCa channels in salivary gland.

Biochemical and biophysical research communications·2025
Same author

Protocadherin alpha gene cluster variants are potentially associated with short root anomaly in Japanese.

European journal of orthodontics·2025

Related Experiment Video

Updated: May 9, 2026

Development of Amelogenin-chitosan Hydrogel for In Vitro Enamel Regrowth with a Dense Interface
08:26

Development of Amelogenin-chitosan Hydrogel for In Vitro Enamel Regrowth with a Dense Interface

Published on: July 10, 2014

Amelogenins: Multi-Functional Enamel Matrix Proteins and Their Binding Partners.

Naoto Haruyama1, Junko Hatakeyama, Keiji Moriyama

  • 1GCOE Program, International Research Center for Molecular Science in Tooth and Bone Diseases Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8549, Japan ; Maxillofacial Orthognathics, Department of Maxillofacial Reconstruction and Function Division of Maxillofacial/Neck Reconstruction, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8549, Japan.

Journal of Oral Biosciences
|August 6, 2013
PubMed
Summary

Amelogenins, key enamel proteins, also function in hard tissue formation and signaling. Research shows they can regenerate periodontal tissues and prevent root resorption, suggesting new therapeutic uses.

Keywords:
M180amelogeninleucine-rich amelogenin peptide(LRAP)signaling moleculesyeast two-hybrid

More Related Videos

Accessing the Cytotoxicity and Cell Response to Biomaterials
09:46

Accessing the Cytotoxicity and Cell Response to Biomaterials

Published on: July 8, 2021

Micro-dissection of Enamel Organ from Mandibular Incisor of Rats Exposed to Environmental Toxicants
08:12

Micro-dissection of Enamel Organ from Mandibular Incisor of Rats Exposed to Environmental Toxicants

Published on: March 29, 2018

Related Experiment Videos

Last Updated: May 9, 2026

Development of Amelogenin-chitosan Hydrogel for In Vitro Enamel Regrowth with a Dense Interface
08:26

Development of Amelogenin-chitosan Hydrogel for In Vitro Enamel Regrowth with a Dense Interface

Published on: July 10, 2014

Accessing the Cytotoxicity and Cell Response to Biomaterials
09:46

Accessing the Cytotoxicity and Cell Response to Biomaterials

Published on: July 8, 2021

Micro-dissection of Enamel Organ from Mandibular Incisor of Rats Exposed to Environmental Toxicants
08:12

Micro-dissection of Enamel Organ from Mandibular Incisor of Rats Exposed to Environmental Toxicants

Published on: March 29, 2018

Area of Science:

  • Dental research
  • Biochemistry
  • Cell signaling

Background:

  • Amelogenins are primary enamel matrix proteins crucial for tooth development.
  • Recent findings reveal amelogenins in mesenchymal tissues, suggesting broader roles beyond enamel formation.
  • Enamel matrix derivatives containing amelogenins promote periodontal regeneration.

Purpose of the Study:

  • To explore the signaling roles of amelogenins (M180 and LRAP) in mesenchymal cells.
  • To identify protein-binding partners of leucine-rich amelogenin peptide (LRAP).
  • To understand amelogenin's role in hard tissue formation and disease prevention.

Main Methods:

  • Literature review on amelogenin functions.
  • Analysis of therapeutic applications of enamel matrix derivatives.
  • Yeast two-hybrid assay to identify LRAP-binding proteins.

Main Results:

  • Amelogenins (M180, LRAP) regulate cementoblast/PDL cell behavior in vitro.
  • Amelogenin deficiency leads to increased osteoclastogenesis and root resorption.
  • Recombinant amelogenins inhibit osteoclastogenesis in vivo and in vitro.

Conclusions:

  • Amelogenins act as signaling molecules in mesenchymal cells, influencing hard tissue formation and resorption.
  • Further research into amelogenin signaling pathways may yield novel treatments for dental diseases.
  • Amelogenins show potential in preventing idiopathic root resorption and promoting periodontal regeneration.