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Related Experiment Videos

C1q receptor on murine cells.

A Erdei1

  • 1Basel Institute for Immunology, Switzerland.

Journal of Immunology (Baltimore, Md. : 1950)
|September 15, 1990
PubMed
Summary
This summary is machine-generated.

Murine B cells and macrophages bind the C1q subcomponent, with varying capacity across different cell types. These C1q-binding molecules on murine cells are antigenically similar to human C1q receptors.

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Area of Science:

  • Immunology
  • Cell Biology

Background:

  • The complement system plays a crucial role in innate and adaptive immunity.
  • C1q is the initiating component of the classical complement pathway.
  • Understanding C1q-cell interactions is vital for immunomodulation.

Purpose of the Study:

  • To investigate the binding capacity of the C1q subcomponent to various murine cells.
  • To identify and characterize C1q-binding molecules on murine cells.
  • To assess the antigenic relationship between murine and human C1q receptors.

Main Methods:

  • Cytofluorometric analysis using biotinylated human C1q and streptavidin-FITC.
  • In vitro cell activation assays.
  • C1q affinity chromatography followed by SDS-PAGE.

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  • Immunological cross-reactivity using a polyclonal antibody against human C1q receptor.

    • Main Results:

    • Splenocytes and thymocytes showed the majority of C1q-reactive cells in B cell and macrophage populations.
    • Activated B cell blasts demonstrated significant C1q binding, while T cell blasts did not.
    • Pre-B lymphomas and myeloid cell lines exhibited strong C1q binding (90-98%).
    • SDS-PAGE identified C1q-binding molecules at approximately 80 kDa, with additional bands at 50 and 45 kDa in splenocytes and B cell blasts.
    • A polyclonal antibody against human C1q receptor cross-reacted with murine C1q-binding molecules.

    Conclusions:

    • Murine B cells and macrophages are primary C1q-binding cells.
    • C1q-binding capacity varies significantly with cell type and activation status.
    • Murine C1q receptors share antigenic similarities with their human counterparts.