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Characterization of immune complexes by isoelectric focusing in agarose gels
W L Hoffman1, P J Kelly, M Larrumbide
1Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas.
Journal of Immunological Methods
|August 28, 1990
Abstract:
A method is described for the characterization of immune complexes on thin-layer agarose isoelectric focusing (IEF) gels. This method involves dissociating immune complexes and then maintaining this dissociation during IEF in agarose gels containing 9 M urea. After IEF, the immune complex components can be quantitatively transferred to nitrocellulose in less than 15 min, and a variety of immunostaining procedures can be used to probe these blotted components. No loss of biological activity was detected in any of the blotted components.