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Biobanking of Human Aqueous and Vitreous Liquid Biopsies for Molecular Analyses
Published on: September 11, 2023
Characterizing the normal proteome of human ciliary body
Renu Goel1,2, Krishna R Murthy1,3,4, Srinivas M Srikanth1,5
1Institute of Bioinformatics, International Technology Park, Bangalore 560 066, India.
Clinical Proteomics
|August 7, 2013
Summary
This study presents the largest proteomic analysis of the human ciliary body, identifying 2,815 proteins. Over 95% of these proteins are newly discovered, offering crucial insights into aqueous humor secretion and eye diseases like glaucoma.
Area of Science:
- Ophthalmology
- Proteomics
- Molecular Biology
Background:
- The ciliary body is vital for aqueous humor production, lens support, and accommodation.
- It is a key target for glaucoma medications, influencing intraocular pressure.
- Previous molecular studies of the human ciliary body have been limited in scale.
Purpose of the Study:
- To conduct a large-scale proteomic investigation of the normal human ciliary body.
- To identify novel proteins within the ciliary body.
- To establish a foundational proteomic dataset for understanding ciliary body function and related diseases.
Main Methods:
- Liquid chromatography-tandem mass spectrometry (LC-MS/MS) based proteomic analysis.
- Proteomic profiling of normal human ciliary body tissue.
- Comparative analysis with plasma proteome.
- Pathway enrichment analysis.
Main Results:
- Identification of 2,815 proteins in the human ciliary body.
- Discovery of 896 proteins unique to the ciliary body.
- Identification of novel proteins including IPO5, ATL2, BCAP29, BSG, CAPN1, CPNE6, FBLN1, and LGALS1.
- Proteins were significantly associated with the ubiquitin pathway, EIF2 signaling, glycolysis, and gluconeogenesis.
Conclusions:
- This study provides the largest protein catalog of the human ciliary body to date.
- Over 95% of identified proteins are novel to the ciliary body proteome.
- These findings enhance understanding of aqueous humor secretion and anterior segment eye diseases like glaucoma and presbyopia.
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