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Related Experiment Video

Updated: May 9, 2026

Development of an In Vitro Assay to Quantitate Hematopoietic Stem and Progenitor Cells (HSPCs) in Developing Zebrafish Embryos
10:28

Development of an In Vitro Assay to Quantitate Hematopoietic Stem and Progenitor Cells (HSPCs) in Developing Zebrafish Embryos

Published on: November 30, 2017

Assaying hematopoiesis using zebrafish.

Sonja Boatman1, Francesca Barrett, Sruthi Satishchandran

  • 1Stem Cell Program and Division of Hematology/Oncology, Children's Hospital Boston, Harvard Medical School, Boston, MA, USA.

Blood Cells, Molecules & Diseases
|August 7, 2013
PubMed
Summary
This summary is machine-generated.

Zebrafish models advance hematopoiesis research. Recent progress in genetic and chemical screens, alongside cell sorting and transplantation, enhances understanding of blood cell development and disease treatment.

Keywords:
AGMALMBMCHTENUFACSFSCGVHDHCTHematopoiesisICMMHCPAFPGPLMPol II Associated FactorSSCScreensStem cellTGFβTIF1γTransplantationWKMZebrafishanterior lateral mesodermaorta–gonad–mesonephrosbone marrowcaudal hematopoietic tissueethylnitrosoureafluorescence-activated cell sortingforward light scattergraft-versus-host-diseasehCBhematopoietic cellular transplantationhours post fertilizationhpfhuman cord bloodintermediate cell massmajor histocompatibility complexposterior lateral mesodermprostaglandinside light scattertranscription intermediary factor1γtransforming growth factor betawhole kidney marrow

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Last Updated: May 9, 2026

Development of an In Vitro Assay to Quantitate Hematopoietic Stem and Progenitor Cells (HSPCs) in Developing Zebrafish Embryos
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Area of Science:

  • Hematology and developmental biology
  • Comparative genomics and toxicology

Background:

  • Zebrafish are increasingly utilized as a vertebrate model for hematopoiesis research due to their advantageous biological characteristics.
  • Their suitability for high-throughput genetic and chemical screening surpasses that of many other model organisms.

Purpose of the Study:

  • To review recent advancements in zebrafish hematopoiesis research over the last decade.
  • To highlight the integration of various methodologies for functional analysis of hematopoietic lineages.

Main Methods:

  • Review of forward genetic screens and chemical screens.
  • Analysis of fluorescence-activated cell sorting (FACS) data.
  • Evaluation of hematopoietic stem cell transplantation assays.

Main Results:

  • Significant progress has been made in dissecting hematopoietic development and function in zebrafish.
  • Integrated approaches allow for comprehensive functional assays of hematopoietic cells.
  • Zebrafish models facilitate the discovery of novel regulators of hematopoiesis.

Conclusions:

  • Zebrafish research continues to significantly expand knowledge of hematopoiesis.
  • These advancements hold promise for developing novel therapeutic strategies for hematological disorders.