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Published on: February 5, 2015
SbcCD-mediated processing of covalent gyrase-DNA complex in Escherichia coli
Sandra Aedo1, Yuk-Ching Tse-Dinh
1Department of Biochemistry & Molecular Biology, New York Medical College, Valhalla, New York, USA.
Abstract:
Quinolones trap the covalent gyrase-DNA complex in Escherichia coli, leading to cell death. Processing activities for trapped covalent complex have not been characterized. A mutant strain lacking SbcCD nuclease activity was examined for both accumulation of gyrase-DNA complex and viability after quinolone treatment. Higher complex levels were found in ΔsbcCD cells than in wild-type cells after incubation with nalidixic acid and ciprofloxacin. However, SbcCD activity protected cells against the bactericidal action of nalidixic acid but not ciprofloxacin.
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