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Transforming Static Barrier Tissue Models into Dynamic Microphysiological Systems
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A contact line pinning based microfluidic platform for modelling physiological flows.

Chih-kuan Tung1, Oleh Krupa, Elif Apaydin

  • 1Department of Biological and Environmental Engineering, Cornell University, Ithaca, NY, USA. ct348@cornell.edu mw272@cornell.edu.

Lab on a Chip
|August 7, 2013
PubMed
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This study presents a novel microfluidic platform using contact line pinning to create realistic 3D environments. This enables the study of how interstitial and intramural flows affect cancer cell behavior and motility.

Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Microfluidics

Background:

  • Studying cellular behavior in 3D microenvironments requires advanced microfluidic platforms.
  • Existing methods often lack the ability to precisely control fluid flow dynamics within complex matrices.
  • Understanding interstitial and intramural flows is crucial for modeling physiological processes like tumor cell migration.

Purpose of the Study:

  • To develop and validate a microfluidic platform for generating controlled interstitial and intramural flows.
  • To investigate the impact of these flows on breast tumor cell (MDA-MB-231) morphology and motility.
  • To provide a framework for studying cellular behavior in physiologically relevant 3D co-culture settings.

Main Methods:

  • Utilized a contact line pinning technique to confine collagen biomatrix in wall-less microfluidic channels.

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  • Engineered an endothelial cell monolayer to form a vascular vessel mimic for intramural flow studies.
  • Created a 3D collagen matrix with embedded cells for interstitial flow experiments.

    • Main Results:

    • Successfully generated stable, wall-less endothelial tubes and uniform interstitial flows within a 3D collagen matrix.
    • Demonstrated that interstitial flows significantly modulate breast tumor cell morphology and motility.
    • Observed enhanced motility in a sub-population of tumor cells due to interstitial flow.

    Conclusions:

    • The contact line pinning microfluidic platform effectively recreates controlled interstitial and intramural flow conditions in a 3D microenvironment.
    • This platform is valuable for studying cell transmigration, growth, and adhesion under physiologically relevant conditions.
    • The findings highlight the influence of microenvironment flow dynamics on cancer cell behavior.