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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Mass Cytometry: Protocol for Daily Tuning and Running Cell Samples on a CyTOF Mass Cytometer
10:59

Mass Cytometry: Protocol for Daily Tuning and Running Cell Samples on a CyTOF Mass Cytometer

Published on: November 2, 2012

A practical guide to multiplexed mass cytometry.

Nevena Zivanovic1, Andrea Jacobs, Bernd Bodenmiller

  • 1Institute of Molecular Life Sciences, University of Zürich, 8057, Zürich, Switzerland.

Current Topics in Microbiology and Immunology
|August 7, 2013
PubMed
Summary

Mass cytometry, using inductively coupled plasma mass spectrometry (ICP-MS), allows detailed single-cell analysis. Mass-tag cellular barcoding (MCB) multiplexes samples for higher throughput and improved data quality in cell research.

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Area of Science:

  • Biotechnology
  • Cellular Biology
  • Analytical Chemistry

Background:

  • Inductively coupled plasma mass spectrometry (ICP-MS) advances have expanded mass cytometry's use in life sciences.
  • Mass cytometry allows high-dimensional single-cell analysis by measuring metal-tagged antibodies.
  • Understanding single-cell biology in complex samples requires scalable analysis methods.

Purpose of the Study:

  • To introduce mass-tag cellular barcoding (MCB) for upscaling mass cytometry to screening approaches.
  • To enable simultaneous analysis of multiple cell samples using MCB.
  • To describe MCB implementation in a 96-well format for medium-scale experiments.

Main Methods:

  • MCB utilizes 'n' metal ion tags to multiplex up to 2^n samples.
  • Each cell sample is labeled with a unique mass barcode.
  • Combined samples undergo a single immunostaining and mass cytometry analysis.

Main Results:

  • MCB significantly increases sample throughput.
  • MCB reduces the consumption of expensive antibodies.
  • MCB enhances overall data quality in mass cytometry experiments.

Conclusions:

  • MCB is a valuable cell-based multiplexing technique for mass cytometry.
  • MCB facilitates medium-scale profiling and screening experiments.
  • MCB optimizes resource utilization and data acquisition in cellular analysis.