Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 9, 2026

Rapid Glyco-Qualitative Assessment of Recombinant Proteins Using a Fully Automated System
05:19

Rapid Glyco-Qualitative Assessment of Recombinant Proteins Using a Fully Automated System

Published on: June 28, 2024

Automated, high-throughput IgG-antibody glycoprofiling platform.

Henning Stöckmann1, Barbara Adamczyk, Jerrard Hayes

  • 1NIBRT GlycoScience Group, NIBRT- The National Institute for Bioprocessing Research and Training , Fosters Avenue, Mount Merrion, Blackrock, Co. Dublin, Ireland.

Analytical Chemistry
|August 8, 2013
PubMed
Summary

Researchers developed a high-throughput, automated platform for analyzing N-glycans on immunoglobulin G (IgG) antibodies. This cost-effective glycomics tool enhances biomarker discovery and therapeutic antibody monitoring.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structural implications of glycosylation on the voltage-gated sodium channel β3-subunit.

Journal of structural biology·2026
Same author

Endometriosis specific vaginal microbiota links to urine and serum N-glycome.

Scientific reports·2024
Same author

Dietary amino acids, macronutrients, vaginal birth, and breastfeeding are associated with the vaginal microbiome in early pregnancy.

Microbiology spectrum·2024
Same author

Erythropoietin <i>N</i>-glycosylation of Therapeutic Formulations Quantified and Characterized: An Interlab Comparability Study of High-Throughput Methods.

Biomolecules·2024
Same author

N-glycans from serum IgG and total serum glycoproteins specific for endometriosis.

Scientific reports·2023
Same author

CHOmpact: A reduced metabolic model of Chinese hamster ovary cells with enhanced interpretability.

Biotechnology and bioengineering·2023

Area of Science:

  • Biochemistry
  • Immunology
  • Analytical Chemistry

Background:

  • Decoding complex carbohydrate functions in biological systems remains a significant challenge.
  • Immunoglobulin G (IgG) antibodies are crucial in immune responses and biopharmaceutical development.
  • High-throughput glycomics data is essential for understanding glycosylation patterns.

Purpose of the Study:

  • To develop a robotized, low-cost platform for high-throughput N-glycan analysis of IgG antibodies.
  • To facilitate glycoprofiling for biomarker discovery and therapeutic antibody monitoring.
  • To improve the efficiency and reproducibility of glycomics data generation.

Main Methods:

  • Automated sample preparation using a robotic liquid-handling workstation.
  • IgG affinity purification, denaturation, and glycan release on a multiwell filtration device.

More Related Videos

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
13:21

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Published on: May 4, 2012

Profiling Anti-Neu5Gc IgG in Human Sera with a Sialoglycan Microarray Assay
09:13

Profiling Anti-Neu5Gc IgG in Human Sera with a Sialoglycan Microarray Assay

Published on: July 13, 2017

Related Experiment Videos

Last Updated: May 9, 2026

Rapid Glyco-Qualitative Assessment of Recombinant Proteins Using a Fully Automated System
05:19

Rapid Glyco-Qualitative Assessment of Recombinant Proteins Using a Fully Automated System

Published on: June 28, 2024

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
13:21

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Published on: May 4, 2012

Profiling Anti-Neu5Gc IgG in Human Sera with a Sialoglycan Microarray Assay
09:13

Profiling Anti-Neu5Gc IgG in Human Sera with a Sialoglycan Microarray Assay

Published on: July 13, 2017

  • Glycan purification via solid-supported hydrazide followed by ultra-performance liquid chromatography (UPLC) quantification.

    • Main Results:

    • Developed a robotized platform for N-glycan analysis of IgG.
    • Achieved high-throughput preparation of 96 samples in 22 hours with excellent reproducibility.
    • Demonstrated a cost-effective approach for glycomics data generation.

    Conclusions:

    • The automated platform significantly facilitates biomarker discovery.
    • Enables efficient glycosylation monitoring of therapeutic IgGs.
    • Advances high-throughput glycomics analysis for biological and biopharmaceutical applications.