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Proposed reclassification of Pasteurella lymphangitidis Sneath & Stevens 1990 as Yersinia pseudotuberculosis

Olivier Gaillot1, Olivier Lemenand2, Michaël Marceau3

  • 1Lille University Medical Centre, F-59037 Lille, France.

International Journal of Systematic and Evolutionary Microbiology
|August 8, 2013
PubMed

Insights

Pasteurella lymphangitidis shares identical 16S rRNA gene sequences with Yersinia species. This bacterium should be reclassified as Yersinia pseudotuberculosis due to phage susceptibility and invasin gene presence.

Area of Science:

  • Microbiology
  • Bacterial Taxonomy
  • Molecular Biology

Background:

  • Pasteurella lymphangitidis and Yersinia species are distinct bacterial taxa.
  • Accurate bacterial classification is crucial for understanding disease transmission and developing effective treatments.

Purpose of the Study:

  • To investigate the taxonomic relationship between Pasteurella lymphangitidis, Yersinia pseudotuberculosis, and Yersinia pestis.
  • To determine if Pasteurella lymphangitidis should be reclassified based on molecular and genetic evidence.

Main Methods:

  • 16S rRNA gene sequencing was performed for Pasteurella lymphangitidis, Yersinia pseudotuberculosis, and Yersinia pestis.
  • Multilocus sequence analysis (MLSA) was employed to differentiate the species.
  • Susceptibility to a Yersinia pseudotuberculosis-specific phage was tested.
  • The presence of the Yersinia pseudotuberculosis-specific invasin gene was assessed in Pasteurella lymphangitidis.

Main Results:

  • 16S rRNA gene sequences of all three species were found to be identical.
  • Multilocus sequence analysis failed to distinguish between Pasteurella lymphangitidis, Yersinia pseudotuberculosis, and Yersinia pestis.
  • Pasteurella lymphangitidis exhibited susceptibility to a Yersinia pseudotuberculosis phage.
  • The invasin gene, specific to Yersinia pseudotuberculosis, was detected in Pasteurella lymphangitidis.

Conclusions:

  • The genetic and phenotypic data strongly support the reclassification of Pasteurella lymphangitidis.
  • Pasteurella lymphangitidis should be taxonomically reclassified as Yersinia pseudotuberculosis.
  • This reclassification clarifies the phylogenetic position and potential pathogenicity of this bacterium.

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