Related Experiment Video

Updated: May 9, 2026

Tissue Engineering of Tumor Stromal Microenvironment with Application to Cancer Cell Invasion
05:48

Tissue Engineering of Tumor Stromal Microenvironment with Application to Cancer Cell Invasion

Published on: March 18, 2014

Procollagen III N-terminal propeptide and desmosine are released by matrix destruction in pulmonary tuberculosis

Jo Seddon1, Victoria Kasprowicz, Naomi F Walker

  • 1Infectious Diseases and Immunity.

The Journal of Infectious Diseases
|August 8, 2013
PubMed
Abstract

Insights

Matrix metalloproteinases (MMPs) generate matrix degradation products (MDPs) in tuberculosis lung disease. These circulating MDPs, like procollagen III N-terminal propeptide (PIIINP), correlate with lung pathology and can help diagnose tuberculosis.

Area of Science:

  • Pulmonary Medicine
  • Immunopathology
  • Biochemistry

Background:

  • Tuberculosis (TB) transmission occurs via pulmonary disease patients.
  • Matrix metalloproteinases (MMPs) are implicated in lung destruction during TB.
  • Matrix degradation products (MDPs) resulting from MMP activity in TB have not been well-studied.

Purpose of the Study:

  • To investigate if MMP activity generates matrix turnover products (MDPs) that correlate with lung pathology in tuberculosis.
  • To assess the diagnostic potential of MDPs and MMPs in pulmonary tuberculosis.

Main Methods:

  • Prospective collection of induced sputum and plasma from HIV-positive and negative pulmonary TB patients and controls.
  • Analysis of MDPs and MMP concentrations using ELISA and Luminex array in two distinct patient cohorts.

Main Results:

  • Procollagen III N-terminal propeptide (PIIINP) and desmosine were significantly elevated in the sputum of HIV-uninfected TB patients.
  • Plasma PIIINP levels correlated with sputum MMP-1 and radiological scores, indicating reflection of lung destruction.
  • Plasma PIIINP and MMP-8 concentrations were higher in TB patients, achieving an AUC of 0.832 for diagnosis.

Conclusions:

  • Matrix metalloproteinase-driven immunopathology in pulmonary tuberculosis generates measurable matrix degradation products.
  • Circulating MDPs, particularly PIIINP, serve as valuable biomarkers for lung destruction in TB.
  • Combined analysis of PIIINP and MMP-8 shows promise for diagnosing pulmonary tuberculosis.
Keywords:
extracellular matriximmunopathologylungmatrix metalloproteinasemycobacteria

Related Concept Videos

Pulmonary Tuberculosis II01:28

Pulmonary Tuberculosis II

Tuberculosis, or TB, is a bacterial infectious disease caused by Mycobacterium tuberculosis. While its primary impact is on the lungs, leading to pulmonary tuberculosis, it can also affect various other organs, a condition referred to as extrapulmonary tuberculosis.
Here is a detailed explanation of its pathophysiology:
Transmission: The process begins when a person inhales droplet nuclei containing M. tuberculosis. These are typically released into the air when an individual with pulmonary or...
Type IV Collagen of Basal Lamina01:05

Type IV Collagen of Basal Lamina

Type IV collagen is a 400 nm long, network-forming collagen that acts as a barrier between the epithelial and endothelial cells. Type IV collagen  forms the backbone of the basement membrane by scaffolding with laminin, entactin, proteoglycans, and fibronectin. Apart from rendering structural support to the basement membrane, it also helps entail signaling potentials necessary for both pathological and physiological functions.
A type IV collagen molecule has six alpha chains which can exist in...
Pulmonary Tuberculosis III01:31

Pulmonary Tuberculosis III

Tuberculosis (TB) is a contagious infection primarily affecting the lung parenchyma but which can also affect other body parts. TB can be classified based on disease development, presentation, and the affected anatomical site.
The first classification is based on the development of the disease, and it includes the following categories:
Role of Matrix Metalloproteases in Degradation of ECM01:23

Role of Matrix Metalloproteases in Degradation of ECM

Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult body.
A...
Pulmonary Tuberculosis IV01:26

Pulmonary Tuberculosis IV

Tuberculosis, more commonly referred to as TB, is an infectious disease stemming from Mycobacterium tuberculosis. While it primarily impacts the lungs, TB can also affect other body areas. Given its severity and global impact, timely and accurate diagnosis is crucial for controlling its spread and improving patient outcomes.
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
Phases of Wound Repair01:28

Phases of Wound Repair

Following injury, the integrity of the injured tissues must be reestablished. For example, in skin tissue, wound repair involves coordination among resident skin cells, blood mononuclear cells, extracellular matrix, growth factors, and cytokines to complete the healing cascade.
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...