Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
Since the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dendritic Cell α-Ketoglutarate Regulates Tfh Polarization in Allergy.

Allergy·2026
Same author

Small cell carcinoma in a bladder diverticulum: a rare case report and literature review.

Frontiers in oncology·2026
Same author

Structural characterization and functional evaluation of deer sinew peptide-calcium chelate for intestinal calcium transport and osteogenic differentiation.

International journal of biological macromolecules·2026
Same author

A high-density SNP linkage map reveals a major LG3 QTL hotspot controlling early-maturity-related traits in ridge gourd (<i>Luffa acutangula</i>).

Frontiers in plant science·2026
Same author

Multimodal sequencing identifies synergistic mechanisms driving resistance to neoadjuvant nivolumab treatment in hepatocellular carcinoma.

Molecular cancer·2026
Same author

Synthesis of 1,2,4-Triazole Derivatives Via Three-Component Tandem Cyclization Reactions Based on N-N Bond Formation.

Organic letters·2026

Related Experiment Video

Updated: May 9, 2026

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp
10:44

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp

Published on: June 20, 2018

Analysis of unannotated equine transcripts identified by mRNA sequencing.

Stephen J Coleman1, Zheng Zeng, Matthew S Hestand

  • 1Maxwell H. Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, Kentucky, United States of America.

Plos One
|August 8, 2013
PubMed
Summary
This summary is machine-generated.

Researchers discovered four novel equine-specific gene transcripts using RNA-sequencing. These transcripts, potentially involved in transcriptional regulation, show unique genomic features in horses.

More Related Videos

Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood
09:40

Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood

Published on: November 28, 2018

Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples
11:23

Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples

Published on: December 22, 2014

Related Experiment Videos

Last Updated: May 9, 2026

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp
10:44

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp

Published on: June 20, 2018

Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood
09:40

Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood

Published on: November 28, 2018

Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples
11:23

Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples

Published on: December 22, 2014

Area of Science:

  • Genomics
  • Comparative genomics
  • Equine genetics

Background:

  • RNA sequencing (RNA-seq) of equine mRNA identified numerous transcripts without prior annotation.
  • The potential exists to discover novel, equine-specific gene structures beyond known homologs.

Purpose of the Study:

  • To identify and characterize novel equine gene transcripts.
  • To investigate the potential for equine-specific gene structures and functions.

Main Methods:

  • RNA-sequencing (RNA-seq) to identify transcripts.
  • Bioinformatic filtering based on expression, genomic location, and exon number.
  • Sanger sequencing of RT-PCR amplicons for validation.
  • Conserved domain searches for functional prediction.
  • Comparative analysis of synteny and sequence conservation with other mammalian genomes.

Main Results:

  • 428 putative equine transcripts were identified, unmapped to known genes.
  • 36 transcripts were prioritized, with four selected for detailed investigation.
  • Sanger sequencing confirmed expression and structure of the four selected transcripts.
  • One transcript, found in the cerebellum, possesses a kruppel-associated box (KRAB) domain, suggesting a role in transcriptional regulation.
  • Comparative genomic analysis revealed approximately 73% conserved synteny but also localized regions of low conservation and sequence inversion.

Conclusions:

  • The four investigated transcripts are likely equine-specific.
  • These findings contribute to understanding equine genomic diversity and novel gene discovery.
  • The KRAB-domain-containing transcript offers insights into potential equine-specific regulatory mechanisms.