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Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis
Published on: March 7, 2018
An integrated peptide-antigen microarray on plasmonic gold films for sensitive human antibody profiling
Bo Zhang1, Justin A Jarrell, Jordan V Price
1Department of Chemistry, Stanford University, Stanford, California, United States of America.
Plos One
|August 8, 2013
Summary
This study introduces a highly sensitive peptide microarray using nanostructured gold for enhanced fluorescence, improving peptide-antibody interaction screening. This new platform aids in diagnosing systemic lupus erythematosus (SLE) by profiling antibodies against histone peptides and antigens.
Area of Science:
- Biotechnology
- Nanotechnology
- Proteomics
Background:
- High-throughput screening of peptide-antibody interactions is crucial for various biological and medical applications.
- Current peptide microarrays lack the sensitivity for detecting low-abundance proteins or weak interactions.
- Nanostructured plasmonic substrates offer potential for enhancing detection sensitivity.
Purpose of the Study:
- To develop a novel peptide microarray platform with ultrahigh sensitivity for peptide-antibody interaction screening.
- To create an integrated array for profiling human antibodies in systemic lupus erythematosus (SLE) patients.
- To assess the diagnostic potential of combined peptide and whole antigen profiling for SLE.
Main Methods:
- Fabrication of a peptide microarray on nanostructured plasmonic gold substrates.
- Utilizing metal-enhanced Near-Infrared fluorescence enhancement (NIR-FE) for signal amplification.
- Development of an integrated array combining histone peptides (modified and unmodified) and whole antigens.
- Profiling human antibodies in sera from SLE patients and healthy controls.
Main Results:
- The nanostructured plasmonic gold substrate achieved hundreds-fold NIR-FE, enabling ultrahigh-sensitivity screening.
- The integrated array successfully profiled antibodies against histone peptides and whole antigens in SLE patient sera.
- A panel of biomarkers targeting modified/unmodified histone peptides and whole antigens demonstrated superior accuracy in differentiating SLE patients from healthy individuals compared to single targets.
Conclusions:
- The developed plasmonic peptide microarray platform significantly enhances sensitivity for peptide-antibody interaction detection.
- Integrated profiling of antibodies against histone peptides and whole antigens shows promise as a more accurate diagnostic biomarker panel for SLE.
- This technology has broad implications for proteomic analysis, drug discovery, and biomarker identification.
