Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Typical Model Studies01:30

Typical Model Studies

Fluid mechanics model studies often utilize scaled-down systems to predict fluid behavior in full-scale environments, such as river flows, dam spillways, and structures interacting with open surfaces. Maintaining Froude number similarity in river models is crucial, as it replicates surface flow features like wave patterns and velocities.
Dynamic Equilibrium02:20

Dynamic Equilibrium

A reversible chemical reaction represents a chemical process that proceeds in both forward (left to right) and reverse (right to left) directions. When the rates of the forward and reverse reactions are equal, the concentrations of the reactant and product species remain constant over time and the system is at equilibrium. A special double arrow is used to emphasize the reversible nature of the reaction. The relative concentrations of reactants and products in equilibrium systems vary greatly;...
Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Electrochemical Systems01:24

Electrochemical Systems

Electrochemical systems provide a fascinating insight into the dynamic interplay of charged species within various phases. One notable example is the interaction between a membrane permeable to K⁺ ions but not to Cl⁻ ions, separating an aqueous KCl solution from pure water. As K⁺ ions diffuse through the membrane, they generate net charges on each phase, leading to a potential difference between them.Similarly, when a piece of Zn is immersed in an aqueous ZnSO₄ solution, the Zn metal, composed...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Assembly and phylogenetic analysis of the mitochondrial genome of endangered medicinal plant Huperzia crispata.

Functional & integrative genomics·2023
Same author

Genomic diversity and evolution analysis of severe fever with thrombocytopenia syndrome in East Asia from 2010 to 2022.

Frontiers in microbiology·2023
Same author

Advances and challenges in biotechnological production of chondroitin sulfate and its oligosaccharides.

International journal of biological macromolecules·2023
Same author

lncRNA ARAP1-AS1 enhances proliferation and impairs apoptosis of lymphoma cells by sponging miR-6867-5p.

Cancer biomarkers : section A of Disease markers·2023
Same author

Pharmacological effects and mechanism of Kaihoujian Throat Spray (children's type) in the treatment of pediatric acute pharyngitis and tonsillitis.

Heliyon·2023
Same author

Computational Quantification of Cancer Immunoediting.

Cancer immunology research·2023

Related Experiment Video

Updated: May 9, 2026

Finite Element Modelling of a Cellular Electric Microenvironment
08:23

Finite Element Modelling of a Cellular Electric Microenvironment

Published on: May 18, 2021

Mathematical model and dynamic computer simulation on free flow zone electrophoresis.

Jie Zhang1, Jian Yan, Si Li

  • 1Laboratory of Bioseparation and Analytical Biochemistry, State Key Laboratory of Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China. cxcao@sjtu.edu.cn.

The Analyst
|August 8, 2013
PubMed
Summary
This summary is machine-generated.

A new mathematical model and software simulate free-flow zone electrophoresis (FFZE) separation processes. This tool accurately predicts FFZE outcomes, optimizes experimental conditions, and determines solute properties, validating experimental results.

More Related Videos

Microfluidic Device for the Separation of Non-Metastatic (MCF-7) and Non-Tumor (MCF-10A) Breast Cancer Cells Using AC Dielectrophoresis
08:33

Microfluidic Device for the Separation of Non-Metastatic (MCF-7) and Non-Tumor (MCF-10A) Breast Cancer Cells Using AC Dielectrophoresis

Published on: August 11, 2022

Precise Electrochemical Sizing of Individual Electro-Inactive Particles
05:03

Precise Electrochemical Sizing of Individual Electro-Inactive Particles

Published on: August 4, 2023

Related Experiment Videos

Last Updated: May 9, 2026

Finite Element Modelling of a Cellular Electric Microenvironment
08:23

Finite Element Modelling of a Cellular Electric Microenvironment

Published on: May 18, 2021

Microfluidic Device for the Separation of Non-Metastatic (MCF-7) and Non-Tumor (MCF-10A) Breast Cancer Cells Using AC Dielectrophoresis
08:33

Microfluidic Device for the Separation of Non-Metastatic (MCF-7) and Non-Tumor (MCF-10A) Breast Cancer Cells Using AC Dielectrophoresis

Published on: August 11, 2022

Precise Electrochemical Sizing of Individual Electro-Inactive Particles
05:03

Precise Electrochemical Sizing of Individual Electro-Inactive Particles

Published on: August 4, 2023

Area of Science:

  • Analytical Chemistry
  • Separation Science
  • Computational Chemistry

Background:

  • Free-flow zone electrophoresis (FFZE) is a powerful separation technique.
  • Accurate simulation of FFZE processes is crucial for experimental optimization and understanding solute behavior.
  • Existing models may lack comprehensive simulation capabilities for FFZE dynamics and parameter optimization.

Purpose of the Study:

  • To develop a mathematical model for calculating physico-chemical parameters in FFZE.
  • To create user-friendly computer software based on the model for FFZE simulation.
  • To validate the software's accuracy and reliability through experimental comparison.

Main Methods:

  • Development of a comprehensive mathematical model integrating zone electrophoresis, electrolyte solution, hydrodynamics, diffusion, and conversion equations.
  • Implementation of the model into computer software using Delphi XE2.
  • Experimental validation of simulation results against real-world FFZE experiments and literature data.

Main Results:

  • The developed software accurately simulates dynamic FFZE processes.
  • The software allows for the simulation and optimization of key operational parameters (electric field, flow rate, pH).
  • The simulator successfully predicts electropherograms for multiple analytes and can convert FFZE electropherograms to capillary zone electrophoresis format.
  • Simulations showed high agreement with experimental data and references, confirming software reliability.

Conclusions:

  • The mathematical model and software provide a reliable tool for FFZE simulation and analysis.
  • The developed system aids in optimizing experimental conditions and determining fundamental physico-chemical parameters.
  • This work significantly advances the application and understanding of free-flow zone electrophoresis.