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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Three Different Protocols of Corneal Collagen Crosslinking in Keratoconus: Conventional, Accelerated and Iontophoresis
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Proteomic and gene expression patterns of keratoconus.

Arkasubhra Ghosh1, Lei Zhou, Anuprita Ghosh

  • 1Genes, Repair and Regeneration at Ophthalmic Workstation (GROW Research Laboratory), Narayana Nethralaya, Bangalore, Karnataka, India.

Indian Journal of Ophthalmology
|August 9, 2013
PubMed
Summary

Keratoconus involves complex gene and protein changes in the cornea, leading to ectasia. This study identifies deregulated molecules in keratoconus epithelium and tears, offering new diagnostic and management insights.

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Area of Science:

  • Ophthalmology and Vision Science
  • Molecular Biology
  • Genetics

Background:

  • Keratoconus is a progressive corneal disease characterized by thinning and ectasia.
  • The precise molecular mechanisms, including gene and protein expression changes, driving keratoconus pathology remain incompletely understood.
  • Understanding these molecular alterations is crucial for developing effective diagnostic and therapeutic strategies.

Purpose of the Study:

  • To investigate differential gene and protein expression in keratoconus corneas compared to healthy controls.
  • To identify specific molecular pathways and proteins involved in the pathogenesis of keratoconus.
  • To explore potential novel biomarkers for clinical diagnosis and management of keratoconus.

Main Methods:

  • Microarray analysis of total ribonucleic acid (RNA) from corneal epithelium of keratoconus patients and controls.
  • Whole proteome analysis of corneal epithelium and tears from keratoconus patients and healthy individuals.
  • Comparative analysis to identify deregulated genes and proteins.

Main Results:

  • Identification of a number of deregulated structural proteins, signaling molecules, cytokines, proteases, and enzymes in keratoconus corneas.
  • Significant differences in gene and protein expression profiles between keratoconus and healthy corneal samples.
  • Data suggest alterations in cellular structure, signaling, and extracellular matrix remodeling in keratoconus.

Conclusions:

  • The study provides insights into the complex molecular biology of keratoconus, highlighting key deregulated pathways.
  • Identified molecular changes offer potential targets for novel diagnostic tools and therapeutic interventions for keratoconus.
  • Further research into these gene and protein expression differences can advance the understanding and clinical management of keratoconus.