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Related Experiment Video

Updated: May 9, 2026

Whole Mount Imaging to Visualize and Quantify Peripheral Lens Structure, Cell Morphology, and Organization
05:45

Whole Mount Imaging to Visualize and Quantify Peripheral Lens Structure, Cell Morphology, and Organization

Published on: January 19, 2024

Lens density tracking in mice by Scheimpflug imaging.

Oliver Puk1, Martin Hrabĕ de Angelis, Jochen Graw

  • 1German Mouse Clinic, Institute of Developmental Genetics, Helmholtz Zentrum München, German Research Center for Environmental Health (GmbH), Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany. oliver.puk@helmholtz-muenchen.de

Mammalian Genome : Official Journal of the International Mammalian Genome Society
|August 10, 2013
PubMed
Summary

Scheimpflug imaging reveals lifelong lens opacification in mice, with C57BL/6J mice showing the highest transparency. This technique is crucial for detecting subtle differences in cataract development across various mouse strains.

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Area of Science:

  • Ophthalmology
  • Animal Models
  • Biomedical Imaging

Background:

  • Scheimpflug imaging is a novel technique for in vivo anterior eye segment imaging.
  • It allows for quantitative determination of lens transparency in mice.
  • This is essential for studying cataract formation in mouse models, including longitudinal studies.

Purpose of the Study:

  • To assess age-related changes in lens transparency across common laboratory mouse inbred lines using Scheimpflug imaging.
  • To identify disease-associated irregularities and strain-specific differences in cataract development.
  • To determine the suitability of different mouse strains for cataract research using this imaging modality.

Main Methods:

  • Scheimpflug measurements were performed on five common laboratory inbred mouse lines (C57BL/6J, C3HeB/FeJ, FVB/NCrl, BALB/cByJ, and 129/SvJ) between 2 and 12 months of age.

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Last Updated: May 9, 2026

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  • Quantitative lens densitometry was used to assess lens transparency.
  • Age-related changes and strain-specific opacities were analyzed.

    • Main Results:

    • Progressive cortical lens opacification was observed in most strains, with early onset in C57BL/6J, C3HeB/FeJ, and 129/SvJ mice.
    • Nuclear opacities developed in these strains as well.
    • C57BL/6J mice exhibited the lowest mean lens densities, indicating higher transparency.
    • Strain-specific anterior lens opacities and corneal lesions were identified in certain strains.
    • Lens density significantly increased between 6 and 12 months in most strains, except 129/SvJ.

    Conclusions:

    • Lifelong opacification processes occur in the mouse lens.
    • Scheimpflug imaging is highly sensitive, detecting differences in lens transparency below 1%.
    • C57BL/6J mice are most suitable for cataract research due to their high lens transparency and dark coat color, minimizing light reflection interference.
    • This imaging method effectively differentiates cataract development across mouse strains.