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Related Experiment Video

Updated: May 9, 2026

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

Published on: December 29, 2015

Junonia coenia Densovirus (JcDNV) Genome Structure.

Hanh T Pham¹, Oanh T H Huynh, Françoise-Xavière Jousset

¹INRS-Institut Armand-Frappier, Laval, QC, Canada.

Genome Announcements

|August 10, 2013

Summary

This summary is machine-generated.

The first published Junonia coenia densovirus genome sequence was inaccurate. New sequencing confirmed its close relationship to other densoviruses, correcting previous errors.

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Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes

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Published on: July 27, 2018

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Last Updated: May 9, 2026

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

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Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes

Published on: July 27, 2018

Area of Science:

Virology
Molecular Biology
Genomics

Background:

The initial genome sequence of Junonia coenia densovirus, the first published densovirus genome, contained errors.
These errors included incomplete inverted terminal repeats and ambiguous nucleotides/indels.
This led to an inaccurate map of the virus's open reading frames.

Purpose of the Study:

To re-sequence and accurately characterize the complete genome of Junonia coenia densovirus.
To correct the previously published genome sequence and open reading frame map.
To clarify the phylogenetic position of Junonia coenia densovirus within the Densovirus genus.

Main Methods:

Whole genome sequencing of cloned Junonia coenia densovirus DNA.
Bioinformatic analysis to assemble the genome sequence.
Comparative genomic analysis with other densoviruses.

Main Results:

A complete and accurate genome sequence for Junonia coenia densovirus was determined.
The corrected sequence resolved issues with inverted terminal repeats and ambiguous nucleotides.
Phylogenetic analysis revealed Junonia coenia densovirus is closely related to other viruses in the Densovirus genus.

Conclusions:

The corrected Junonia coenia densovirus genome sequence provides a reliable basis for future research.
This study refines our understanding of densovirus genome structure and evolution.
The findings support the close evolutionary relationship between Junonia coenia densovirus and its relatives.