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Orthogonal test design for optimizing the extraction of total flavonoids from Inula helenium
Chun-Yan Guo1, Jin Wang, Yong Hou
1Department of Graduate, HeBei Medical University, Shijiazhuang 050017, Hebei, China.
Background:
Inula helenium, which belongs to thecomposite family, is an important crude drug in traditional Chinese medicine.
Materials And Methods:
The effects of ethanol concentration, liquid to solid ratio, extraction temperature, and duration of microwave irradiation on the flavonoid extraction yield were studied through a single-factor experiment. An orthogonal array (L9(3(4))) was then constructed to achieve the best extraction conditions.
Results:
Variance analysis revealed that ethanol concentration significantly affected the extraction yield. The optimal conditions were as follows: ethanol concentration, 50% (v/v); liquid to solid ratio, 15:1; duration of microwave irradiation, 240 s; and extraction temperature, 60°C.
Conclusion:
Under these optimal conditions, the total flavonoid yield was 18.34 ± 0.64 mg/g. The use of a microwave-assisted process dramatically reduced the time needed for extraction of flavonoids from I. helenium.
