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Labeling Cytosolic Targets in Live Cells with Blinking Probes
Jianmin Xu1, Jason Chang, Qi Yan
1Department of Chemistry & Biochemistry, University of California Los Angeles, Los Angeles CA 90095.
Abstract:
With the advent of superresolution imaging methods, fast dynamic imaging of biological processes in live cells remains a challenge. A subset of these methods requires the cellular targets to be labeled with spontaneously blinking probes. The delivery and specific targeting of cytosolic targets and the control of the probes' blinking properties are reviewed for three types of blinking probes: quantum dots, synthetic dyes, and fluorescent proteins.
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