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Characterization and expression analysis of common carp Cyprinus carpio TLR5M
Duo Duan1, Zhen Sun, Shengmei Jia
1Key Laboratory for Zoonosis, Ministry of Education, Institute of Zoonosis, Jilin University , Changchun, People's Republic of China .
DNA and Cell Biology
|August 13, 2013
Summary
The Toll-like receptor 5 (TLR5M) gene in common carp plays a key role in innate immunity. Its expression significantly increases in response to bacterial flagellin and Aeromonas hydrophila infection, crucial for fish defense.
Area of Science:
- Immunology
- Aquatic Biology
- Molecular Biology
Background:
- Toll-like receptor 5 (TLR5) recognizes bacterial flagellin in vertebrates.
- Understanding fish immune responses is vital for aquaculture and conservation.
Purpose of the Study:
- To clone and characterize the TLR5M gene in common carp.
- To investigate the expression patterns and immune-related functions of TLR5M.
Main Methods:
- Rapid amplification of cDNA ends (RACE) for gene cloning.
- Quantitative real-time polymerase chain reaction (qPCR) for gene expression analysis.
- Genomic DNA amplification to determine gene structure.
Main Results:
- The TLR5M cDNA sequence was 3182 bp, encoding an 885-amino acid protein.
- TLR5M showed broad tissue distribution, with highest expression in the liver.
- Gene expression significantly increased in response to flagellin stimulation and Aeromonas hydrophila infection.
- Modulation of TLR5M and cytokine genes (IFN-γ, IL-1β, IL-10, TNF-α4) observed in peripheral blood leucocytes.
Conclusions:
- The TLR5M gene is crucial for the innate immune response in common carp.
- TLR5M activation is involved in recognizing bacterial pathogens and initiating immune signaling pathways.
- Findings provide insights into fish immune mechanisms against bacterial invasion.
