Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Overview of Electron Microscopy01:25

Overview of Electron Microscopy

The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Coordinated chromosome motion emerges from mechanical coupling mediated by the physical spindle environment.

Molecular biology of the cell·2026
Same author

Multiscale temporal tuning of force generation complex machinery governs cortical microtubule interactions during the first mitotic division in <i>C. elegans</i>.

bioRxiv : the preprint server for biology·2026
Same author

The Role of Transient Crosslinks in the Chromatin Search Response to DNA Damage.

International journal of molecular sciences·2025
Same author

Arabidopsis AUGMIN8 Contains Two Independent Microtubule Association Domains.

Cytoskeleton (Hoboken, N.J.)·2025
Same author

Transverse cortical microtubule arrays form persistent unipolar domains in hypocotyl cells of <i>Arabidopsis thaliana</i>.

Molecular biology of the cell·2025
Same author

Three types of actomyosin rings within a common cytoplasm exhibit distinct modes of contractility.

Molecular biology of the cell·2025
Same journal

Quantification of cell viability by automated analysis of live cell imaging.

Methods in cell biology·2026
Same journal

Flow cytometry evaluation of cytotoxicity exerted by effector immune cells against tumor cells.

Methods in cell biology·2026
Same journal

Time-lapse confocal laser scanning microscopy analysis of FOOD formation.

Methods in cell biology·2026
Same journal

Screening and identification of protein-protein interaction using proximity labeling.

Methods in cell biology·2026
Same journal

Quantitative high-content profiling of mitochondrial morphology with automated statistical analysis and integrated data visualization.

Methods in cell biology·2026
Same journal

Super-resolution imaging of cell death in Drosophila tissues via expansion and pan-expansion microscopy.

Methods in cell biology·2026
See all related articles

Related Experiment Video

Updated: May 9, 2026

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
10:28

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

A high-resolution multimode digital microscope system.

Edward D Salmon1, Sidney L Shaw, Jennifer C Waters

  • 1Department of Biology, University of North Carolina, Chapel Hill, North Carolina, USA.

Methods in Cell Biology
|August 13, 2013
PubMed
Summary
This summary is machine-generated.

A new high-resolution digital imaging system combines a Nikon microscope, Hamamatsu CCD camera, and MetaMorph software for advanced cellular imaging. This system enhances studies of microtubules, mitosis, and live-cell dynamics.

Keywords:
Charge-coupled deviceGreen fluorescent proteinHamamatsuMetaMorph digital imaging systemNikon FXA microscope

More Related Videos

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging
13:49

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

Published on: January 11, 2011

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
16:10

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins

Published on: March 22, 2012

Related Experiment Videos

Last Updated: May 9, 2026

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
10:28

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging
13:49

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

Published on: January 11, 2011

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
16:10

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins

Published on: March 22, 2012

Area of Science:

  • Microscopy and Digital Imaging
  • Cell Biology
  • Biophysics

Background:

  • High-resolution imaging is crucial for understanding complex cellular processes.
  • Existing systems may lack the multimode capabilities for diverse cellular studies.

Purpose of the Study:

  • To develop and describe a high-resolution, multimode digital imaging system.
  • To detail the design criteria and components of the integrated system.

Main Methods:

  • Integration of a Nikon FXA microscope, Hamamatsu C4880 cooled CCD camera, and MetaMorph digital imaging software.
  • Utilized wide-field epifluorescent and transmitted light microscopy.
  • Incorporated advanced optics, focus control, and vibration isolation.

Main Results:

  • The system achieves high-resolution imaging in both epifluorescent and transmitted light modes without specimen manipulation.
  • Demonstrated applicability to microtubule and mitosis studies, live-cell protein tracking (e.g., green fluorescent protein fusions), and endoplasmic reticulum dynamics.
  • Enabled correlation of microtubule assembly dynamics with membrane and cell cortex dynamics in migrating epithelial cells.

Conclusions:

  • The developed digital imaging system offers a versatile platform for advanced cellular and subcellular imaging.
  • Its specialized design facilitates detailed analysis of dynamic cellular processes and genetic studies in yeast.