Jove
Visualize
Contact Us

Related Concept Videos

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Defining the host dependencies and the transcriptional landscape of RSV infection.

mBio·2025
Same author

Defining the host dependencies and the transcriptional landscape of RSV infection and bystander activation.

bioRxiv : the preprint server for biology·2025
Same author

MADDD-seq, a novel massively parallel sequencing tool for simultaneous detection of DNA damage and mutations.

Nucleic acids research·2024
Same author

Early, precise, and safe clinical evaluation of the pharmacodynamic effects of novel agents in the intact human tumor microenvironment.

Frontiers in pharmacology·2024
Same author

Systematic functional interrogation of SARS-CoV-2 host factors using Perturb-seq.

Nature communications·2023
Same author

Broad susceptibility of <i>Candida auris</i> strains to 8-hydroxyquinolines and mechanisms of resistance.

mBio·2023
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 9, 2026

Oncogenic Gene Fusion Detection Using Anchored Multiplex Polymerase Chain Reaction Followed by Next Generation Sequencing
09:49

Oncogenic Gene Fusion Detection Using Anchored Multiplex Polymerase Chain Reaction Followed by Next Generation Sequencing

Published on: July 5, 2019

Simultaneous digital quantification and fluorescence-based size characterization of massively parallel sequencing

Matthew T Laurie1, Jessica A Bertout, Sean D Taylor

  • 1Translational Research Program, Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA.

Biotechniques
|August 13, 2013
PubMed
Summary

Droplet digital PCR (ddPCR) offers a streamlined approach for sequencing library quality control. This method accurately quantifies and determines DNA fragment size in a single assay, improving library preparation reliability.

More Related Videos

Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples
07:30

Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples

Published on: June 8, 2020

Related Experiment Videos

Last Updated: May 9, 2026

Oncogenic Gene Fusion Detection Using Anchored Multiplex Polymerase Chain Reaction Followed by Next Generation Sequencing
09:49

Oncogenic Gene Fusion Detection Using Anchored Multiplex Polymerase Chain Reaction Followed by Next Generation Sequencing

Published on: July 5, 2019

Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples
07:30

Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples

Published on: June 8, 2020

Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • Accurate quantification and fragment size determination are critical for successful massively parallel sequencing (next-generation sequencing) library preparation.
  • Current quality control methods, such as qPCR and electrophoresis, are time-consuming and have limitations affecting library calibration reliability.

Purpose of the Study:

  • To introduce and validate droplet digital PCR (ddPCR) as an alternative method for sequencing library quality control.
  • To demonstrate a novel ddPCR assay for simultaneous DNA quantification and fragment size determination.

Main Methods:

  • Exploited a correlation between droplet fluorescence intensity and amplicon size.
  • Developed a single ddPCR assay for joint quantification and size determination of target DNA in sequencing libraries.

Main Results:

  • The proposed ddPCR method achieves accurate and precise joint quantification and size determination of DNA.
  • This approach offers a more reliable alternative to traditional library quality control methods.

Conclusions:

  • ddPCR provides an efficient and accurate solution for sequencing library quality control.
  • This method enhances the reliability of library preparation, reducing costs and improving data yields for next-generation sequencing applications.