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Naloxone antagonizes behavioural and ECoG effects induced by systemic or intracerebral administration of some
1Istituto di Farmacologia, Facoltà di Medicina di Catanzaro, Università degli Studi, Reggio Calabria, Italy.
Rat interferon, alpha-interferon, interleukin-2 and recombinant interleukin-2 microinjected into the third cerebral ventricle produced in rats typical behavioural sedation and/or sleep and ECoG synchronization while beta-interferon produced no behavioural sleep and ECoG synchronization. A slight sedation was observed after highest dose of beta-interferon only. During sleep induced by these lymphokines a dose-dependent increase in total voltage power as well as in the 0.5-3, 4-7 and 12-16 Hz frequency bands was observed. Much lower doses were required to produce similar behavioural and ECoG spectrum power effects after microinfusion of interferons and interleukin-2 into the locus coeruleus. No significant behavioural and ECoG changes were obtained after microinfusion of the same doses of interferons and interleukin-2 into other areas of the brain (caudate nucleus, dorsal hippocampus, substantia nigra-pars compacta-, ventromedial hypothalamus). The behavioural and ECoG effects of alpha-interferon, rat interferon and interleukin-2 were blocked in animals pretreated with naloxone. These results are consistent with the hypothesis that the behavioural and ECoG effects of these lymphokines are mediated at locus coeruleus level via a stimulation of opiate receptors.
Rat interferon, alpha-interferon, interleukin-2 and recombinant interleukin-2 microinjected into the third cerebral ventricle produced in rats typical behavioural sedation and/or sleep and ECoG synchronization while beta-interferon produced no behavioural sleep and ECoG synchronization. A slight sedation was observed after highest dose of beta-interferon only. During sleep induced by these lymphokines a dose-dependent increase in total voltage power as well as in the 0.5-3, 4-7 and 12-16 Hz frequency bands was observed. Much lower doses were required to produce similar behavioural and ECoG spectrum power effects after microinfusion of interferons and interleukin-2 into the locus coeruleus. No significant behavioural and ECoG changes were obtained after microinfusion of the same doses of interferons and interleukin-2 into other areas of the brain (caudate nucleus, dorsal hippocampus, substantia nigra-pars compacta-, ventromedial hypothalamus). The behavioural and ECoG effects of alpha-interferon, rat interferon and interleukin-2 were blocked in animals pretreated with naloxone. These results are consistent with the hypothesis that the behavioural and ECoG effects of these lymphokines are mediated at locus coeruleus level via a stimulation of opiate receptors.