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Published on: October 9, 2016
In vitro evaluation of DispersinB on methicillin-resistant Staphylococcus pseudintermedius biofilm
Ryen Turk1, Ameet Singh, Joyce Rousseau
1Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G2W1, Canada. rturk@uoguelph.ca
Abstract:
Methicillin resistant Staphylococcus pseudintermedius (MRSP) is an important canine pathogen that has been shown to produce biofilm in vitro. Biofilm production may be an important virulence factor and methods to eliminate biofilm-associated infections are required. An enzyme of Aggregatibacter actinomycetemcomitans, DispersinB, has been shown to degrade the extracellular matrix in the biofilm of a variety of bacteria including Staphylococcus aureus and Staphylococcus epidermidis. The objective of this study was to determine the effect of DispersinB on MRSP biofilm production and eradication in vitro. A quantitative microtitre plate assay was used to assess the impact of DispersinB on 30 MRSP isolates from dogs. While DispersinB did not have any effect on MRSP growth (P=0.98), it reduced biofilm formation (P=0.0002) and degraded established biofilm (P=0.0001). These data indicate that in vivo study of the effect of this enzyme in indicated to determine if it may be a useful treatment option for MRSP biofilm-associated infections.
Insights
DispersinB enzyme effectively reduces methicillin-resistant Staphylococcus pseudintermedius (MRSP) biofilm formation and degrades existing biofilms in canine isolates. Further in vivo studies are recommended for this potential MRSP infection treatment.
Area of Science:
- Veterinary Microbiology
- Infectious Diseases
- Biotechnology
Background:
- Methicillin-resistant Staphylococcus pseudintermedius (MRSP) is a significant canine pathogen.
- MRSP exhibits biofilm production in vitro, potentially contributing to virulence.
- Effective methods for eliminating MRSP biofilm infections are needed.
Purpose of the Study:
- To investigate the efficacy of DispersinB enzyme against MRSP biofilm production and established biofilms.
- To assess DispersinB's impact on MRSP isolates from canine infections.
Main Methods:
- Quantitative microtitre plate assays were employed.
- Thirty MRSP isolates from dogs were tested.
- The effect of DispersinB on MRSP growth, biofilm formation, and degradation was evaluated.
Main Results:
- DispersinB did not significantly affect MRSP growth (P=0.98).
- DispersinB significantly reduced MRSP biofilm formation (P=0.0002).
- DispersinB significantly degraded established MRSP biofilms (P=0.0001).
Conclusions:
- DispersinB demonstrates potent anti-biofilm activity against MRSP in vitro.
- The findings suggest DispersinB as a potential therapeutic agent for MRSP biofilm-associated infections.
- Further in vivo studies are warranted to confirm its clinical utility.
