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Updated: May 9, 2026

Protocols for Microapplicator-assisted Infection of Lepidopteran Larvae with Baculovirus
Published on: August 23, 2008
Interactions between an injected polydnavirus and per os baculovirus in gypsy moth larvae
V D'Amico1, J D Podgwaite, R Zerillo
1USDA Forest Service, Northern Research Station, Department of Entomology & Wildlife Ecology, 531 South College Ave, Newark, DE 19716, United States.
Abstract:
Larval gypsy moths, Lymantria dispar (Lepidoptera:Lymantriidae) were co-infected with the L. dispar nucleopolyhedrovirus (LdMNPV) and the Cotesia melanoscela (Hymenoptera:Braconidae) polydnavirus (CmeBV). CmeBV was given along with a parasitoid egg and calyx products in a stinging event, or in the form of an injection of calyx-derived extract. LdMNPV was delivered per os, integrated into artificial diet. Mortality from all sources was recorded over the subsequent three-week period. Neither parasitism nor injections of purified CmeBV with toxin had any effect on the amount of mortality caused by concurrent challenges with LdMNPV.

