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Updated: May 9, 2026

Preparation of Mechanically Stable Self-Assembled Peptides Hydrogels
05:24

Preparation of Mechanically Stable Self-Assembled Peptides Hydrogels

Published on: September 6, 2024

Combining self-assembling peptide gels with three-dimensional elastomer scaffolds.

A Vallés-Lluch1, M Arnal-Pastor, C Martínez-Ramos

  • 1Center for Biomaterials and Tissue Engineering, Universitat Politècnica de València, Cno. de Vera s/n, 46022 Valencia, Spain.

Acta Biomaterialia
|August 13, 2013
PubMed
Summary
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This study combines porous scaffolds with self-assembling peptide (SAP) gels for tissue engineering. The novel scaffold-cum-gel constructs enhance cell colonization, offering a promising platform for advanced tissue regeneration applications.

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Tissue engineering requires advanced scaffolds that mimic the extracellular matrix.
  • Combining porous scaffolds with self-assembling peptide (SAP) gels presents unique challenges and opportunities.
  • Poly(ethyl acrylate) scaffolds and RAD16-I SAP gel were selected for this study.

Purpose of the Study:

  • To investigate the combination of porous scaffolds and SAP gels for tissue engineering.
  • To characterize the in situ gelation, morphology, physicochemical, and mechanical properties of scaffold-cum-gel constructs.
  • To assess the incorporation and release of active molecules and evaluate cell colonization.

Main Methods:

  • In situ gelation of RAD16-I SAP gel within poly(ethyl acrylate) scaffolds.
Keywords:
Cell seedingControlled releasePoly(ethyl acrylate)ScaffoldSelf-assembling peptide gel

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Culturing Mammalian Cells in Three-dimensional Peptide Scaffolds
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Culturing Mammalian Cells in Three-dimensional Peptide Scaffolds

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Last Updated: May 9, 2026

Preparation of Mechanically Stable Self-Assembled Peptides Hydrogels
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Preparation of Mechanically Stable Self-Assembled Peptides Hydrogels

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Culturing Mammalian Cells in Three-dimensional Peptide Scaffolds
07:52

Culturing Mammalian Cells in Three-dimensional Peptide Scaffolds

Published on: June 13, 2018

  • Morphological, physicochemical, and mechanical characterization of constructs.
  • Incorporation and release studies of bovine serum albumin (BSA).
  • Cell seeding and colonization studies using L929 fibroblasts and ovine adipose-derived stem cells.
  • Comparison of static and dynamic cell seeding methods.

    • Main Results:

    • Successful in situ gelation of SAP within scaffold pores.
    • Scaffold-cum-gel constructs exhibited favorable characteristics.
    • BSA incorporation and controlled release were demonstrated.
    • SAP gel significantly improved cell colonization uniformity and density.
    • Dynamically assisted seeding enhanced cell distribution.

    Conclusions:

    • Scaffold-cum-gel constructs provide a cell-friendly environment and structural support for tissue engineering.
    • These constructs show potential for advanced applications requiring controlled cell placement and tissue growth.
    • The combination strategy offers a versatile platform for regenerative medicine.
    • Further preclinical studies are warranted for specific applications.