Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...
Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Zirconium ferrite nanoparticles as smart materials for energy and environmental applications: fractional-order supercapacitors, reservoirs of F<sup>-</sup> ions, and efficient electrocatalysts for water splitting.

Nanoscale advances·2025
Same author

Dual-Baseline Search for Active-to-Sterile Neutrino Oscillations in NOvA.

Physical review letters·2025
Same author

Search for CP-Violating Neutrino Nonstandard Interactions with the NOvA Experiment.

Physical review letters·2024
Same author

Observation of Medium-Induced Yield Enhancement and Acoplanarity Broadening of Low-p_{T} Jets from Measurements in pp and Central Pb-Pb Collisions at sqrt[s_{NN}]=5.02  TeV.

Physical review letters·2024
Same author

Photoproduction of K^{+}K^{-} Pairs in Ultraperipheral Collisions.

Physical review letters·2024
Same author

First Measurement of the |t| Dependence of Incoherent J/ψ Photonuclear Production.

Physical review letters·2024
Same journal

Integration of electrochemical sensors in organ-on-a-chip microfluidic platforms: Advances and perspectives.

Biosensors & bioelectronics·2026
Same journal

DNN-PURE: A deep neural network approach to paper-based urea sensing.

Biosensors & bioelectronics·2026
Same journal

Rationally architected MOF-derived Co<sub>3</sub>O<sub>4</sub>@NiMn-LDH hollow heterostructure-based sensor array empowering sensitive detection and discrimination of neurological biomarkers.

Biosensors & bioelectronics·2026
Same journal

Four-in-one multifunctional CoCu-NC@AuPt nanozyme integrated M13 phage-displayed nanobody based multimodal lateral flow immunoassay for bovine lactoferrin detection.

Biosensors & bioelectronics·2026
Same journal

A novel capillary-driven dual-mode imaging flow cytometry system for malaria parasite detection and quantification.

Biosensors & bioelectronics·2026
Same journal

Liver-targeted alkaline phosphatase-activatable fluorescent probe for imaging liver fibrosis and screening anti-fibrotic natural products.

Biosensors & bioelectronics·2026
See all related articles

Related Experiment Video

Updated: May 8, 2026

Detection of Endotoxin in Nano-formulations Using Limulus Amoebocyte Lysate (LAL) Assays
06:15

Detection of Endotoxin in Nano-formulations Using Limulus Amoebocyte Lysate (LAL) Assays

Published on: January 30, 2019

Recent advances in biosensor based endotoxin detection.

A P Das1, P S Kumar, S Swain

  • 1Bioengineering Laboratory, Centre of Biotechnology, Siksha O Anusandhan University, Bhubaneswar, India.

Biosensors & Bioelectronics
|August 13, 2013
PubMed
Summary
This summary is machine-generated.

Rapid endotoxin detection is crucial for healthcare. LAL biosensors offer a reliable, sensitive, and stable method for identifying these bacterial contaminants, improving patient safety.

Keywords:
BiosensorEndotoxinLAL assayLab-on-a-chipOmicsRecombinant factor CSepsis

More Related Videos

Endotoxin Activity Assay for the Detection of Whole Blood Endotoxemia in Critically Ill Patients
06:28

Endotoxin Activity Assay for the Detection of Whole Blood Endotoxemia in Critically Ill Patients

Published on: June 24, 2019

Related Experiment Videos

Last Updated: May 8, 2026

Detection of Endotoxin in Nano-formulations Using Limulus Amoebocyte Lysate (LAL) Assays
06:15

Detection of Endotoxin in Nano-formulations Using Limulus Amoebocyte Lysate (LAL) Assays

Published on: January 30, 2019

Endotoxin Activity Assay for the Detection of Whole Blood Endotoxemia in Critically Ill Patients
06:28

Endotoxin Activity Assay for the Detection of Whole Blood Endotoxemia in Critically Ill Patients

Published on: June 24, 2019

Area of Science:

  • Microbiology
  • Biomedical Engineering
  • Analytical Chemistry

Background:

  • Endotoxins, or pyrogens, are ubiquitous bacterial lipopolysaccharides posing significant health risks due to high mortality and morbidity associated with contamination.
  • Traditional endotoxin detection methods are slow and inefficient, creating a critical need for agile and prompt detection systems in healthcare and biomedical applications.

Purpose of the Study:

  • To provide a comprehensive overview of current and emerging biosensor-based techniques for endotoxin detection.
  • To highlight the advantages of Limulus Amebocyte Lysate (LAL) biosensors, including sensitivity, stability, and reproducibility.
  • To explore the influence of 'omics' technologies as a novel approach for endotoxin detection.

Main Methods:

  • Review of existing literature on endotoxin detection techniques.
  • Classification and epidemiological analysis of endotoxin contamination.
  • Evaluation of commercially available endotoxin detection sensors.
  • Exploration of novel approaches like omics-based detection.

Main Results:

  • LAL biosensors demonstrate high sensitivity, stability, and reproducibility, making them reliable for endotoxin detection.
  • Current traditional methods are time-consuming, underscoring the need for advanced biosensor technologies.
  • Omics technologies present a revolutionary new avenue for endotoxin detection.

Conclusions:

  • Biosensor technology, particularly LAL-based systems, offers a promising solution for rapid and reliable endotoxin detection.
  • Effective endotoxin control is vital across healthcare, pharmaceutical, and biomedical sectors.
  • Future research should focus on leveraging omics and further developing commercial biosensor applications for endotoxin monitoring.