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Related Concept Videos

Enzyme Inhibition01:30

Enzyme Inhibition

Inhibitors are molecules that reduce enzyme activity by binding to the enzyme. In a normally functioning cell, enzymes are regulated by a variety of inhibitors. Drugs and other toxins can also inhibit enzymes. Some inhibitors bind to the enzyme’s active site, while others inhibit enzymatic activity by binding to other sites on the protein structure.
Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...
Feedback Inhibition00:46

Feedback Inhibition

Biochemical reactions are occurring constantly in cells, converting starting substances to different products, usually with the help of enzymes that speed the reactions. Without enzymes, it would take far too long for most reactions to occur to be useful to the cell!
Enzymes02:34

Enzymes

Inside living organisms, enzymes act as catalysts for many biochemical reactions involved in cellular metabolism. The role of enzymes is to reduce the activation energies of biochemical reactions by forming complexes with its substrates. The lowering of activation energies favor an increase in the rates of biochemical reactions.
Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...
Allosteric Regulation01:08

Allosteric Regulation

Allosteric regulation of enzymes occurs when the binding of an effector molecule to a site that is different from the active site causes a change in the enzymatic activity. This alternate site is called an allosteric site, and an enzyme can contain more than one of these sites. Allosteric regulation can either be positive or negative, resulting in an increase or decrease in enzyme activity. Most enzymes that display allosteric regulation are metabolic enzymes involved in the degradation or...
Regulation of Metabolism01:19

Regulation of Metabolism

Cellular needs and conditions vary from cell to cell and change within individual cells over time. For example, the required enzymes and energetic demands of stomach cells are different from those of fat storage cells, skin cells, blood cells, and nerve cells. Furthermore, a digestive cell works much harder to process and break down nutrients during the time that closely follows a meal compared with many hours after a meal. As these cellular demands and conditions vary, so do the amounts and...

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Related Experiment Video

Updated: May 8, 2026

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
08:06

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

Published on: February 1, 2018

Biosensors based on enzyme inhibition.

Fabiana Arduini1, Aziz Amine

  • 1Dipartimento di Scienze e Tecnologie Chimiche, Università di Roma Tor Vergata, Via della Ricerca Scientifica, 00133, Rome, Italy, fabiana.arduini@uniroma2.it.

Advances in Biochemical Engineering/Biotechnology
|August 13, 2013
PubMed
Summary
This summary is machine-generated.

Biosensors using enzyme inhibition offer sensitive analytical tools. Optimizing parameters like enzyme amount and incubation time enhances performance for environmental, food, and pharmaceutical applications.

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Area of Science:

  • Analytical Chemistry
  • Biotechnology
  • Biochemistry

Background:

  • Enzyme inhibition biosensors are valuable analytical tools.
  • Understanding enzymatic kinetics is crucial for biosensor optimization.
  • These biosensors have diverse applications across multiple fields.

Purpose of the Study:

  • To critically describe biosensors based on enzyme inhibition.
  • To detail the assembly and optimization of these biosensors.
  • To highlight their applications in environmental, food, and pharmaceutical analysis.

Main Methods:

  • Critical evaluation of parameters affecting biosensor sensitivity.
  • Analysis of enzyme immobilization techniques and incubation times.
  • Review of enzymatic kinetics for optimization strategies.

Main Results:

  • Key parameters influencing biosensor sensitivity were identified.
  • Enzymatic kinetics provide a framework for rapid biosensor optimization.
  • Demonstrated wide applicability of enzyme inhibition biosensors.

Conclusions:

  • Biosensors based on enzyme inhibition are effective analytical tools.
  • Optimization through understanding enzymatic kinetics leads to improved performance.
  • These biosensors are versatile for environmental, food, and pharmaceutical monitoring.