Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Immunoprecipitation01:20

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Stepwise enzymatic synthesis of stereoregular poly(hydroxyalkanoates) starting from prochiral β-Keto esters.

Bioresource technology·2026
Same author

MartiniSurf: Automated Simulations of Surface-Immobilized Biomolecular Systems with Martini.

Journal of chemical information and modeling·2026
Same author

Mechanistic Determinants of Oriented Enzyme Immobilization from Martini Simulations.

The journal of physical chemistry letters·2026
Same author

A Multi-Functional Heterogeneous Biocatalyst for the Oxygen-Free Oxidative Condensation of Primary Alcohols into β‑Hydroxy Acids.

ACS catalysis·2026
Same author

Design and Fabrication of a Versatile Microreactor to Study Transaminase-Based Heterogeneous Biocatalysts in Flow.

Chembiochem : a European journal of chemical biology·2025
Same author

Insights into the Catalytic Activity of a Metagenome-Derived Urethanase.

Journal of the American Chemical Society·2025

Related Experiment Video

Updated: May 8, 2026

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy
08:34

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy

Published on: February 5, 2020

Glutaraldehyde-mediated protein immobilization.

Fernando López-Gallego1, Jose M Guisán, Lorena Betancor

  • 1Institute of Catalysis, CSIC, CAMPUS UAM-Cantoblanco, Madrid, Spain.

Methods in Molecular Biology (Clifton, N.J.)
|August 13, 2013
PubMed
Summary

This chapter details glutaraldehyde uses for protein immobilization, including covalent attachment to activated matrices and cross-linking protein aggregates. It provides methods for synthesizing supports and examples of enzyme stabilization and attachment.

More Related Videos

Expression, Solubilization, and Purification of Eukaryotic Borate Transporters
08:55

Expression, Solubilization, and Purification of Eukaryotic Borate Transporters

Published on: March 7, 2019

Rapid Freezing using Sandwich Freezing Device for Good Ultrastructural Preservation of Biological Specimens in Electron Microscopy
09:03

Rapid Freezing using Sandwich Freezing Device for Good Ultrastructural Preservation of Biological Specimens in Electron Microscopy

Published on: July 19, 2021

Related Experiment Videos

Last Updated: May 8, 2026

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy
08:34

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy

Published on: February 5, 2020

Expression, Solubilization, and Purification of Eukaryotic Borate Transporters
08:55

Expression, Solubilization, and Purification of Eukaryotic Borate Transporters

Published on: March 7, 2019

Rapid Freezing using Sandwich Freezing Device for Good Ultrastructural Preservation of Biological Specimens in Electron Microscopy
09:03

Rapid Freezing using Sandwich Freezing Device for Good Ultrastructural Preservation of Biological Specimens in Electron Microscopy

Published on: July 19, 2021

Area of Science:

  • Biochemistry
  • Bioconjugation Chemistry
  • Materials Science

Background:

  • Protein immobilization is crucial for enzyme applications and biosensors.
  • Glutaraldehyde is a versatile cross-linking agent widely used in biochemistry.
  • Effective immobilization strategies enhance protein stability and functionality.

Purpose of the Study:

  • To outline diverse methods for protein immobilization using glutaraldehyde.
  • To detail the synthesis of glutaraldehyde-activated supports.
  • To demonstrate glutaraldehyde's utility in cross-linking proteins for enhanced stability and attachment.

Main Methods:

  • Covalent attachment of proteins to glutaraldehyde-activated matrices.
  • Synthesis of glutaraldehyde-activated supports.
  • Cross-linking of protein aggregates and adsorbed proteins using glutaraldehyde.
  • Utilizing glutaraldehyde to bridge lysine and amine groups.

Main Results:

  • Successful covalent immobilization and stabilization of fructosyltransferase.
  • Demonstrated cross-linking of proteins onto amino-activated supports.
  • Provided examples of DAAO immobilization using glutaraldehyde cross-linking.

Conclusions:

  • Glutaraldehyde is an effective reagent for various protein immobilization techniques.
  • Covalent attachment and cross-linking strategies enhance protein stability and application potential.
  • The described methods offer versatile approaches for protein immobilization in biochemical applications.