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Updated: May 8, 2026

Hydrophobic Salt-modified Nafion for Enzyme Immobilization and Stabilization
Published on: July 11, 2012
Immobilization of enzymes on monofunctional and heterofunctional epoxy-activated supports
Cesar Mateo1, Valeria Grazu, Jose M Guisan
1Institute of Catalysis, CSIC, CAMPUS UAM-Cantoblanco, Madrid, Spain.
Abstract:
The immobilization of proteins on epoxy activated supports is discussed in this chapter. Immobilization on epoxy supports is carried out as a two-step mechanism: in the first step the adsorption of the protein is promoted and in the second step the intramolecular covalent linkage among epoxy groups and nucleophiles of the protein is produced. Based on this mechanism of the need of a first adsorption of the protein on the support, different epoxy supports are described. The different supports are able to immobilize proteins through different orientations being obtained catalysts with different properties of activity, stability, and selectivity.
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