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Related Experiment Video

Updated: May 8, 2026

High Throughput Single-cell and Multiple-cell Micro-encapsulation

High Throughput Single-cell and Multiple-cell Micro-encapsulation

Published on: June 15, 2012

Whole cell entrapment techniques.

Jorge A Trelles¹, Cintia W Rivero

¹Laboratorio de Investigaciones en Biotecnología Sustentable, Universidad Nacional de Quilmes, Bernal, Argentina.

Methods in Molecular Biology (Clifton, N.J.)

|August 13, 2013

Summary

Microbial whole cell immobilization, particularly cell entrapment, offers an efficient, stable, and scalable method for bioprocesses. This technique enhances catalyst performance and simplifies separation in industrial applications.

Area of Science:

Biotechnology and Industrial Microbiology
Biocatalysis and Enzyme Engineering

Background:

Microbial whole cells serve as cost-effective, eco-friendly catalysts in pharmaceutical, environmental, and food industries.
Microorganism immobilization is crucial for optimizing bioprocesses under preparative conditions.

Purpose of the Study:

To highlight the advantages of microorganism immobilization for bioprocesses.
To emphasize cell entrapment as a primary immobilization technique.

Main Methods:

Discusses whole cell immobilization as a strategy for bioprocess development.
Focuses on cell entrapment within a rigid, porous network.

Main Results:

Immobilization provides high operational stability and facilitates upstream separation.

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Whole Cell Electrophysiology of Primary Cultured Murine Enterochromaffin Cells

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Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins

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Cell entrapment ensures high immobilization efficiency (often 100%) and protects cells.

The technique allows for substrate and product diffusion, enabling bioprocess scale-up.

Conclusions:

Microorganism immobilization, especially cell entrapment, is a highly effective method for industrial bioprocesses.
This technique offers significant advantages in terms of stability, separation, and scalability.