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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: May 8, 2026

Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types

Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types

Published on: December 18, 2014

Flow cytometry of neural cells.

Geoffrey W Osborne¹

¹Queensland Brain Institute, The University of Queensland, St. Lucia, Australia.

Methods in Molecular Biology (Clifton, N.J.)

|August 13, 2013

Summary

Flow cytometry, a cell analysis technique, is vital in immunology and cancer research. Its application in neuroscience is growing, despite its early potential being recognized decades ago.

Area of Science:

Neuroscience
Cell Biology
Immunology

Background:

Flow cytometry is a powerful technique for analyzing microscopic particles like cells.
It utilizes optical and fluidic systems to detect light scatter and fluorescence from cells.
Established applications include diagnostics for immunological disorders and cancers, and research in hematology and cell biology.

Purpose of the Study:

To highlight the underutilization of flow cytometry in neuroscience research.
To underscore the significant, yet largely untapped, potential of flow cytometry for advancing neuroscience.

Main Methods:

The abstract describes flow cytometry as a method involving sequential interrogation of cells passing an optical point.
Detection relies on scattered light and fluorescent signals.

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Using Fluorescence Activated Cell Sorting to Examine Cell-Type-Specific Gene Expression in Rat Brain Tissue

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Last Updated: May 8, 2026

Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types

Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types

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The Neuroblast Assay: An Assay for the Generation and Enrichment of Neuronal Progenitor Cells from Differentiating Neural Stem Cell Progeny Using Flow Cytometry

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Using Fluorescence Activated Cell Sorting to Examine Cell-Type-Specific Gene Expression in Rat Brain Tissue

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The core technology involves sophisticated optical and fluidic components.

Main Results:

Flow cytometry is widely applied in various scientific fields, including immunology and cancer diagnostics.
Its adoption in neuroscience has been slow, despite its potential being recognized since the 1980s.
There has been a recent, steady increase in interest in flow cytometry for neuroscience.

Conclusions:

Flow cytometry offers substantial potential for neuroscience applications.
Further exploration and adoption of flow cytometry are encouraged to advance the field of neuroscience.