Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sequencing reactions in microtiter plates.

B F Koop1, R K Wilson, C Chen

  • 1Division of Biology, California Institute of Technology, Pasadena 91125.

Biotechniques
|July 1, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Detection of selection signatures in farmed coho salmon (Oncorhynchus kisutch) using dense genome-wide information.

Scientific reports·2021
Same author

Carotenoid pigmentation in salmon: variation in expression at <i>BCO2-l</i> locus controls a key fitness trait affecting red coloration.

Proceedings. Biological sciences·2019
Same author

NORTH AMERICAN BLACK BEAR mtDNA PHYLOGEOGRAPHY: IMPLICATIONS FOR MORPHOLOGY AND THE HAIDA GWAII GLACIAL REFUGIUM CONTROVERSY.

Evolution; international journal of organic evolution·2017
Same author

Identification of olfactory receptor genes in Atlantic salmon Salmo salar.

Journal of fish biology·2012
Same author

Expression of olfactory receptors in different life stages and life histories of wild Atlantic salmon (Salmo salar).

Molecular ecology·2011
Same author

General and family-specific gene expression responses to viral hemorrhagic septicaemia virus infection in rainbow trout (Oncorhynchus mykiss).

Molecular immunology·2011
Same journal

Investigating the interactomic landscape of survival motor neuron (SMN) and the SMNΔ7 truncated protein.

BioTechniques·2026
Same journal

Antigen retrieval-immunofluorescence on free floating sections to visualize the liver lobule and its cellular makeup.

BioTechniques·2026
Same journal

Special approach of droplet digital polymerase chain reaction (ddPCR) for transgene stability of a Chinese hamster ovary (CHO) cell line.

BioTechniques·2026
Same journal

Strand-specific quantification of L1 ORF0 and related transcripts by multiplex reverse transcription with tagged primers.

BioTechniques·2026
Same journal

Why and when should we choose digital PCR?

BioTechniques·2026
Same journal

Quantitative and unbiased lung alveolar septum assessment in an LPS experimental mouse model using 2D-spatial correlation image analysis from hematoxylin and eosin slides.

BioTechniques·2026
See all related articles

This study presents an affordable method for DNA sequencing of 24 M13 clones using microtiter plates. The optimized protocol significantly reduces incubation times for sequencing reactions, making it faster and more accessible for researchers.

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Genomics

Background:

  • DNA sequencing is crucial for genetic research.
  • Standard sequencing methods can be time-consuming and require specialized equipment.
  • High-throughput sequencing is essential for large-scale genetic studies.

Purpose of the Study:

  • To develop a simple, inexpensive, and rapid method for DNA sequencing.
  • To adapt existing sequencing protocols for microtiter plate format.
  • To improve the efficiency of M13 DNA clone sequencing.

Main Methods:

  • A novel method for performing DNA sequencing reactions on 24 single-strand M13 DNA clones was developed.
  • Custom-designed heating blocks were created to fit microtiter plates within standard laboratory heating modules.

Related Experiment Videos

  • Standard fluorescent and radioisotopic sequencing techniques were minimally modified.

    • Main Results:

    • The described method allows for the sequencing of 24 M13 DNA clones simultaneously in a microtiter plate format.
    • The use of specialized heating blocks simplifies and accommodates elevated temperature incubations.
    • Sequencing reactions can be completed in as little as 40 minutes, significantly reducing processing time.

    Conclusions:

    • This method offers a cost-effective and efficient approach to DNA sequencing for M13 clones.
    • The optimized protocol enhances throughput and reduces hands-on time for researchers.
    • The technique is adaptable for both fluorescent and radioisotopic sequencing, broadening its applicability.