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Published on: December 20, 2014
Cep63 and cep152 cooperate to ensure centriole duplication
Nicola J Brown1, Marko Marjanović, Jens Lüders
1Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms, United Kingdom.
Plos One
|August 13, 2013
Summary
Cep63 is vital for reliable centriole duplication in dividing mammalian cells. It works with Cep152 to ensure correct centrosome formation and maintain genome stability.
Area of Science:
- Cell Biology
- Genetics
- Molecular Biology
Background:
- Centrosomes, composed of centrioles and pericentriolar material, are key microtubule organizing centers in animal cells.
- Proper centrosome function is essential for mitotic spindle organization and genome stability, particularly during embryonic development.
- Mutations in centrosome-related genes, like Cep63 and Cep152, are linked to primary microcephaly.
Purpose of the Study:
- To investigate the role of Cep63 in centriole duplication in mammalian cells.
- To elucidate the interaction and interdependence of Cep63 and Cep152 in centrosome localization and function.
- To define the mechanism by which Cep63 and Cep152 cooperate to regulate centriole formation.
Main Methods:
- Investigated Cep63 function in dividing mammalian cells.
- Analyzed the interaction and centrosomal localization of Cep63 and Cep152.
- Examined the cooperative effects of Cep63 and Cep152 on centriole duplication factors, SAS-6 recruitment, and procentriole formation.
Main Results:
- Cep63 ensures reliable centriole duplication in dividing mammalian cells.
- Cep63 and Cep152 interact independently of centrosome localization and require each other for centrosomal localization.
- Cep63 and Cep152 cooperate to promote the accumulation of centriole duplication factors, facilitating procentriole formation.
Conclusions:
- Cep63 is required for maintaining centriole number in dividing mammalian cells.
- Cep63 and Cep152 are interdependent for centrosomal localization and cooperate to ensure efficient centriole duplication.
- This study establishes the order of events in centriole formation, highlighting the roles of Cep63 and Cep152.
Related Concept Videos
02:25Centrosome Duplication
The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
02:25Centrosome Duplication
The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
02:19The Spindle Assembly Checkpoint
The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
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01:13Centrioles and Centrosomes
Most animal cells comprise a pair of centrioles together called a centrosome. The cell duplicates its centrosome and contains two centrosomes side-by-side, which begin to move apart during the prophase. As the centrosomes migrate to two different sides of the cell, microtubules start extending from each centrosome toward the other end. The mitotic spindle is composed of the centrosomes and their emerging microtubules.
Near the end of the prophase, also called late prophase or "prometaphase,"...
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02:20Cohesins
Cohesin protein complexes are a molecular glue that holds two sister chromatids together. They play an important role both in mitosis and meiosis. In mitosis, all cohesin complexes present on the chromosomes are removed before the start of the anaphase stage.
Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of homologous...
Cohesin complexes in Meiotic Division
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02:17Separation of Sister Chromatids
At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
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