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Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
Published on: June 21, 2018
Interleukin-27 rs153109 polymorphism and the risk for immune thrombocytopenia
Haifeng Zhao1, Yizhuo Zhang, Feng Xue
1Department of Hematology and Oncology, TianJin Medical University Cancer Institute and Hospital, Key Laboratory of Cancer Prevention and Therapy , Tianjin , PR China .
Abstract:
The aim of the present study was to investigate the association of single nucleotide polymorphisms (SNP) of the interleukin-27 (IL-27) genes with the risk for immune thrombocytopenia (ITP). In this case-control study, the genotyping of IL-27 rs153109 polymorphism was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). In ITP patients, the frequencies of GG, GA and AA genotypes, and G and A alleles were 9.7%, 58.5%, 31.8%, 38.9% and 61.1%, respectively. There was no significant difference in genotype and alleles distribution between the ITP patient and the healthy controls (p = 0.68 and 0.80, respectively). Similar results were found between the two groups when stratified by the age and disease course including non-chronic adult, chronic adult, non-chronic childhood, and chronic childhood. In conclusion, the IL-27 polymorphism may not be involved in susceptibility to ITP in a Chinese population.
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