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Updated: May 8, 2026

Sampling and Pretreatment of Tooth Enamel Carbonate for Stable Carbon and Oxygen Isotope Analysis
Published on: August 15, 2018
About sound science, stable isotope dilution, blasphemy and heresy
1Active Biotech AB, Box 724, Scheelevagen 22, Lund, SE-220 07, Sweden. leif.svensson@activebiotech.com
Abstract:
The molar ratio between an analyte and its equal labeled with stable isotope(s), used as a bioanalytical IS, is here suggested as an essential (and currently not commonly applied) validation parameter. This parameter, when calculated and measured, gives the possibility for the bioanalyst to compare calibration graphs and QC samples over time independently of added amount of IS. Additionally, when QC samples and calibration samples do not agree it is obvious to identify where the discrepancies are. Whether calibration samples are needed when this parameter is extensively and carefully validated and understood, is also discussed.
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