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Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy

Published on: May 18, 2011

Focusing polychromatic light through strongly scattering media.

Hari P Paudel¹, Chris Stockbridge, Jerome Mertz

¹Boston University Photonics Center, Boston, MA, USA.

|August 14, 2013

Summary

We optimized focusing of polychromatic light through scattering media using feedback, linking focus intensity to speckle contrast. This method enhances light focusing for better imaging in complex materials.

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Area of Science:

Optics and Photonics
Materials Science
Biomedical Imaging

Background:

Light scattering in complex media like biological tissues or colloidal suspensions hinders direct imaging.
Traditional focusing techniques struggle with the dynamic and wavelength-dependent nature of scattering.

Purpose of the Study:

To demonstrate feedback-optimized focusing of spatially coherent polychromatic light through strongly scattering media.
To establish the relationship between optimized focus intensity and initial far-field speckle contrast.
To analyze the influence of feedback signal characteristics on the spectral bandwidth of the focused light.

Main Methods:

Utilized a Micro-Electro-Mechanical Systems (MEMS) spatial light modulator for wavefront shaping.
Employed camera-based and spectrometer-based feedback systems for optimization.
Investigated the spectral correlations within the transmitted light.

Main Results:

Achieved feedback-optimized focusing of polychromatic light in strongly scattering samples.
Demonstrated a direct correlation between optimized focus intensity and initial far-field speckle contrast.
Observed that the spectral bandwidth of the optimized focus is dependent on the feedback signal characteristics.

Conclusions:

The spectral bandwidth modification suggests a change in the number of independent frequency components transmitted by the sample.
A simple model for polychromatic focus enhancement was developed and experimentally validated.
This technique offers a pathway for improved imaging and manipulation of light in scattering environments.