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Related Concept Videos

Atomic Fluorescence Spectroscopy01:29

Atomic Fluorescence Spectroscopy

Atomic fluorescence spectroscopy (AFS) is an analytical technique that involves the electronic transitions of atoms in a flame, furnace, or plasma being excited by electromagnetic (EM) radiation. When these atoms absorb energy, they become excited and subsequently release energy as they return to their original state. This emitted light, or "fluorescence," is observed at a right angle to the incident beam. Both absorption and emission processes transpire at distinct wavelengths, which are...
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High Precision FRET at Single-molecule Level for Biomolecule Structure Determination
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Absolute instrument spectral response measurements using angle-resolved parametric fluorescence.

Feng-Kuo Hsu1, Chih-Wei Lai

  • 1Department of Physics and Astronomy, Michigan State University, East Lansing, Michigan 48824, USA.

Optics Express
|August 14, 2013
PubMed
Summary
This summary is machine-generated.

Broadband parametric fluorescence offers a compact primary light source for measuring instrument spectral efficiency. This study demonstrates its use for spectral response function measurements between 450-1000 nm.

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Area of Science:

  • Optics and Photonics
  • Nonlinear Optics
  • Spectroscopy

Background:

  • Absolute measurements of instrument spectral efficiency traditionally use blackbody sources.
  • Compact and reliable primary light sources are needed for advanced optical metrology.
  • Parametric fluorescence offers a tunable and broadband alternative.

Purpose of the Study:

  • To present a novel setup utilizing broadband parametric fluorescence for absolute spectral efficiency measurements.
  • To characterize the instrument spectral response function in the visible to near-infrared range (450–1000 nm).
  • To validate the experimental results against theoretical predictions.

Main Methods:

  • Utilizing broadband parametric fluorescence from a nonlinear beta-barium borate crystal.
  • Employing angle-resolved imaging spectroscopy.
  • Pumping the nonlinear crystal with a 405-nm diode laser.
  • Comparing experimental angle-resolved spectra with plane-wave theoretical analysis.

Main Results:

  • Successfully generated broadband parametric fluorescence.
  • Measured the instrument spectral response function from 450 to 1000 nm.
  • Experimental spectra and parametric down-conversion efficiency matched theoretical predictions.
  • Demonstrated the feasibility of using parametric fluorescence as a primary source.

Conclusions:

  • Broadband parametric fluorescence is a viable compact primary source for absolute spectral efficiency measurements.
  • The described setup accurately measures instrument spectral response functions.
  • Plane-wave theory provides a good approximation for the observed parametric down-conversion.