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06:06
Variations on Negative Stain Electron Microscopy Methods: Tools for Tackling Challenging Systems
Published on: February 6, 2018
Gradient field microscopy of unstained specimens: reply to comment
Taewoo Kim1, Shamira Sridharan, Gabriel Popescu
1Quantitative Light Imaging Laboratory, Department of Electrical and Computer Engineering, Beckman Institute for Advanced Science & Technology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.
Optics Express
|August 14, 2013
Summary
Fourier filtering enables valuable intrinsic contrast live cell imaging. Our methods effectively utilize sinusoidal masks, addressing comments on approximation limits and demonstrating robust live cell imaging capabilities.
Area of Science:
- Optics and Photonics
- Biomedical Imaging
- Cell Biology
Background:
- Live cell imaging requires high contrast for accurate observation.
- Fourier filtering presents a potential method for enhancing image contrast.
- Previous work by Kim et al. explored Fourier filtering for intrinsic contrast.
Purpose of the Study:
- To respond to comments by Ferrari and Ayubi regarding the paper by Kim et al.
- To reaffirm the effectiveness of Fourier filtering methods for intrinsic contrast in live cell imaging.
- To clarify the application of sinusoidal masks and their relevance to approximation limits.
Main Methods:
- Utilizing Fourier filtering techniques for image processing.
- Employing sinusoidal masks with varying periods.
- Analyzing results presented in Fig. 2 of the original paper.
Main Results:
- Fourier filtering provides valuable intrinsic contrast for live cell imaging.
- The use of sinusoidal masks, even with approximations, is validated by experimental results.
- Demonstrated effectiveness in overcoming limitations discussed in the comment.
Conclusions:
- The Fourier filtering approach offers a robust method for intrinsic contrast live cell imaging.
- The authors' methodology is sound and supported by presented data.
- Further investigation into Fourier filtering applications in live cell imaging is warranted.
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